1thg: Difference between revisions

Revision as of 09:57, 3 May 2008

1.8 ANGSTROMS REFINED STRUCTURE OF THE LIPASE FROM GEOTRICHUM CANDIDUM

OverviewOverview

A lipase from the fungus Geotrichum candidum is one of only three interfacially activated lipases whose structures have been reported to date. We have previously reported the partially refined 2.2 A structure of this enzyme. We have subsequently extended the resolution and here report the fully refined 1.8 A structure of this lipase. The structure observed in the crystal is apparently not the lipolytic conformation, as the active site is not accessible from the surface of the molecule. A single large cavity is found in the interior of the molecule and extends from the catalytic Ser to two surface helices, suggesting that this face may be the region that interacts with the lipid interface. The mobility of local segments on this face is indicated by temperature factors larger than elsewhere in the molecule and by the observation of several residues whose side-chains are discretely disordered. These observations strongly suggest that this portion of the molecule is involved in interfacial and substrate binding, but the exact nature of the conformational changes induced by binding to the lipid interface can not be determined.

About this StructureAbout this Structure

1THG is a Single protein structure. Full crystallographic information is available from OCA.

ReferenceReference

1.8 A refined structure of the lipase from Geotrichum candidum., Schrag JD, Cygler M, J Mol Biol. 1993 Mar 20;230(2):575-91. PMID:8464065 Page seeded by OCA on Sat May 3 09:57:09 2008

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OCA

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 [[Image:1thg.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1thg", creates the "Structure Box" on the page.
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-|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1thg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1thg OCA], [http://www.ebi.ac.uk/pdbsum/1thg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1thg RCSB]</span>
-}}
 '''1.8 ANGSTROMS REFINED STRUCTURE OF THE LIPASE FROM GEOTRICHUM CANDIDUM'''
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 ==About this Structure==
-THG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THG OCA].
+THG is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THG OCA].
 ==Reference==
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 [[Category: Cygler, M.]]
 [[Category: Schrag, J D.]]
-[[Category: hydrolase(carboxylic esterase)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:57:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:56:04 2008''