6neq: Difference between revisions

Latest revision as of 08:28, 21 November 2024

Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit-Class-IIStructure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit-Class-II

6neq, resolution 3.32Å

Structural highlights

6neq is a 10 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.32Å
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RT27_BOVIN

Publication Abstract from PubMed

The human mitochondrial translational initiation factor 3 (IF3mt) carries mitochondrial-specific amino acid extensions at both its N and C termini (N- and C-terminal extensions [NTE and CTE, respectively]), when compared with its eubacterial counterpart. Here we present 3.3- to 3.5-A-resolution cryoelectron microscopic structures of the mammalian 28S mitoribosomal subunit in complex with human IF3mt. Unique contacts observed between the 28S subunit and N-terminal domain of IF3mt explain its unusually high affinity for the 28S subunit, whereas the position of the mito-specific NTE suggests NTE's role in binding of initiator tRNA to the 28S subunit. The location of the C-terminal domain (CTD) clarifies its anti-association activity, whereas the orientation of the mito-specific CTE provides a mechanistic explanation for its role in destabilizing initiator tRNA in the absence of mRNA. Furthermore, our structure hints at a possible role of the CTD in recruiting leaderless mRNAs for translation initiation. Our findings highlight unique features of IF3mt in mitochondrial translation initiation.

Structure of Human Mitochondrial Translation Initiation Factor 3 Bound to the Small Ribosomal Subunit.,Koripella RK, Sharma MR, Haque ME, Risteff P, Spremulli LL, Agrawal RK iScience. 2019 Feb 22;12:76-86. doi: 10.1016/j.isci.2018.12.030. Epub 2019 Jan 3. PMID:30677741^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Koripella RK, Sharma MR, Haque ME, Risteff P, Spremulli LL, Agrawal RK. Structure of Human Mitochondrial Translation Initiation Factor 3 Bound to the Small Ribosomal Subunit. iScience. 2019 Feb 22;12:76-86. doi: 10.1016/j.isci.2018.12.030. Epub 2019 Jan 3. PMID:30677741 doi:http://dx.doi.org/10.1016/j.isci.2018.12.030

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OCA

[1] Koripella RK, Sharma MR, Haque ME, Risteff P, Spremulli LL, Agrawal RK. Structure of Human Mitochondrial Translation Initiation Factor 3 Bound to the Small Ribosomal Subunit. iScience. 2019 Feb 22;12:76-86. doi: 10.1016/j.isci.2018.12.030. Epub 2019 Jan 3. PMID:30677741 doi:http://dx.doi.org/10.1016/j.isci.2018.12.030

[1]

@@ Line 3: / Line 3: @@
 <SX load='6neq' size='340' side='right' viewer='molstar' caption='[[6neq]], [[Resolution|resolution]] 3.32&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6neq]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin], [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NEQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NEQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6neq]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NEQ FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.32&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MRPS27 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6neq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6neq OCA], [https://pdbe.org/6neq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6neq RCSB], [https://www.ebi.ac.uk/pdbsum/6neq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6neq ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6neq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6neq OCA], [http://pdbe.org/6neq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6neq RCSB], [http://www.ebi.ac.uk/pdbsum/6neq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6neq ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/IF3M_HUMAN IF3M_HUMAN]] IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.<ref>PMID:12095986</ref>  [[http://www.uniprot.org/uniprot/RT34_BOVIN RT34_BOVIN]] Required for mitochondrial translation, plays a role in maintaining the stability of the small ribosomal subunit and the 12S rRNA that are required for mitoribosome formation.[UniProtKB:Q9JIK9] [[http://www.uniprot.org/uniprot/PTCD3_BOVIN PTCD3_BOVIN]] Mitochondrial RNA-binding protein that has a role in mitochondrial translation.
+[https://www.uniprot.org/uniprot/RT27_BOVIN RT27_BOVIN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 26: @@
 </SX>
 [[Category: Bos taurus]]
-[[Category: Bovin]]
-[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Agrawal, R]]
+[[Category: Agrawal R]]
-[[Category: Koripella, R]]
+[[Category: Koripella R]]
-[[Category: Sharma, M]]
+[[Category: Sharma M]]
-[[Category: Human mitochondrial translation initiation factor 3]]
-[[Category: Ribosomal protein]]

6neq: Difference between revisions

Latest revision as of 08:28, 21 November 2024

Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit-Class-IIStructure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit-Class-II

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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6neq: Difference between revisions

Latest revision as of 08:28, 21 November 2024

Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit-Class-IIStructure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit-Class-II

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Search