6gh8: Difference between revisions
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==Crystal structure of GP1 domain of Lujo virus in complex with the first CUB domain of neuropilin-2== | ==Crystal structure of GP1 domain of Lujo virus in complex with the first CUB domain of neuropilin-2== | ||
<StructureSection load='6gh8' size='340' side='right' caption='[[6gh8]], [[Resolution|resolution]] 2.44Å' scene=''> | <StructureSection load='6gh8' size='340' side='right'caption='[[6gh8]], [[Resolution|resolution]] 2.44Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6gh8]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6gh8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mammarenavirus_lujoense Mammarenavirus lujoense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GH8 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.44Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gh8 OCA], [https://pdbe.org/6gh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gh8 RCSB], [https://www.ebi.ac.uk/pdbsum/6gh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gh8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/C5ILC1_9VIRU C5ILC1_9VIRU] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 26: | Line 26: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Cohen-Dvashi | [[Category: Mammarenavirus lujoense]] | ||
[[Category: Diskin | [[Category: Cohen-Dvashi H]] | ||
[[Category: Kilimnik | [[Category: Diskin R]] | ||
[[Category: Kilimnik I]] | |||
Latest revision as of 15:41, 6 November 2024
Crystal structure of GP1 domain of Lujo virus in complex with the first CUB domain of neuropilin-2Crystal structure of GP1 domain of Lujo virus in complex with the first CUB domain of neuropilin-2
Structural highlights
FunctionPublication Abstract from PubMedLujo virus (LUJV) has emerged as a highly fatal human pathogen. Despite its membership among the Arenaviridae, LUJV does not classify with the known Old and New World groups of that viral family. Likewise, LUJV was recently found to use neuropilin-2 (NRP2) as a cellular receptor instead of the canonical receptors used by Old World and New World arenaviruses. The emergence of a deadly pathogen into human populations using an unprecedented entry route raises many questions regarding the mechanism of cell recognition. To provide the basis for combating LUJV in particular, and to increase our general understanding of the molecular changes that accompany an evolutionary switch to a new receptor for arenaviruses, we used X-ray crystallography to reveal how the GP1 receptor-binding domain of LUJV (LUJVGP1) recognizes NRP2. Structural data show that LUJVGP1 is more similar to Old World than to New World arenaviruses. Structural analysis supported by experimental validation further suggests that NRP2 recognition is metal-ion dependent and that the complete NRP2 binding site is formed in the context of the trimeric spike. Taken together, our data provide the mechanism for the cell attachment step of LUJV and present indispensable information for combating this phatogen. Structural basis for receptor recognition by Lujo virus.,Cohen-Dvashi H, Kilimnik I, Diskin R Nat Microbiol. 2018 Aug 27. pii: 10.1038/s41564-018-0224-5. doi:, 10.1038/s41564-018-0224-5. PMID:30150732[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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