6by2: Difference between revisions
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<StructureSection load='6by2' size='340' side='right'caption='[[6by2]], [[Resolution|resolution]] 2.35Å' scene=''> | <StructureSection load='6by2' size='340' side='right'caption='[[6by2]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6by2]] is a 3 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6by2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BY2 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6by2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6by2 OCA], [https://pdbe.org/6by2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6by2 RCSB], [https://www.ebi.ac.uk/pdbsum/6by2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6by2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/KCSA_STRCO KCSA_STRCO] Acts as a potassium ion channel (By similarity). | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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*[[Antibody 3D structures|Antibody 3D structures]] | *[[Antibody 3D structures|Antibody 3D structures]] | ||
*[[Potassium channel 3D structures|Potassium channel 3D structures]] | *[[Potassium channel 3D structures|Potassium channel 3D structures]] | ||
*[[3D structures of non-human antibody|3D structures of non-human antibody]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mus musculus]] | ||
[[Category: Cortes DM]] | |||
[[Category: Cortes | [[Category: Cuello LG]] | ||
[[Category: Cuello | [[Category: Labro AJ]] | ||
[[Category: Labro | [[Category: Tilegenova C]] | ||
[[Category: Tilegenova | |||
Latest revision as of 15:29, 6 November 2024
Closed and deep-inactivated conformation of KcsA-T75A mutantClosed and deep-inactivated conformation of KcsA-T75A mutant
Structural highlights
FunctionKCSA_STRCO Acts as a potassium ion channel (By similarity). Publication Abstract from PubMedThe selectivity filter and the activation gate in potassium channels are functionally and structurally coupled. An allosteric coupling underlies C-type inactivation coupled to activation gating in this ion-channel family (i.e., opening of the activation gate triggers the collapse of the channel's selectivity filter). We have identified the second Threonine residue within the TTVGYGD signature sequence of K(+) channels as a crucial residue for this allosteric communication. A Threonine to Alanine substitution at this position was studied in three representative members of the K(+)-channel family. Interestingly, all of the mutant channels exhibited lack of C-type inactivation gating and an inversion of their allosteric coupling (i.e., closing of the activation gate collapses the channel's selectivity filter). A state-dependent crystallographic study of KcsA-T75A proves that, on activation, the selectivity filter transitions from a nonconductive and deep C-type inactivated conformation to a conductive one. Finally, we provide a crystallographic demonstration that closed-state inactivation can be achieved by the structural collapse of the channel's selectivity filter. Inverted allosteric coupling between activation and inactivation gates in K(+) channels.,Labro AJ, Cortes DM, Tilegenova C, Cuello LG Proc Natl Acad Sci U S A. 2018 May 7. pii: 1800559115. doi:, 10.1073/pnas.1800559115. PMID:29735651[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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