1tan: Difference between revisions

Revision as of 09:44, 3 May 2008

TANDEM DNA, NMR, MINIMIZED AVERAGE STRUCTURE

OverviewOverview

The solution structure of an estrone (Es)-tethered tandem DNA duplex consisting of two Es-tethered tetranucleotides and a target octameric DNA sequence is reported. The structure of this Es-tethered tandem duplex has been compared with a corresponding natural tandem duplex without estrones. The Tm of the 3'-Es-tethered tetranucleotide part of the tandem duplex increases by 5 degrees C, whereas the Tm of the 5'-Es-tethered tetranucleotide part increases by 7 degrees C, compared with the corresponding natural counterpart. The NMR structures of both the Es-tethered tandem duplex and the natural counterpart have been based on 24 experimental NMR constraints per residue. Despite the fact that there is considerable distortion at the junction of two Es-tethered tetranucleotides in the major groove of the Es-tethered DNA duplex compared to the natural counterpart, both duplexes do take up B-type DNA structures. It is likely that the spatial proximity of two Es residues, and the resulting hydrophobic interaction between them might be responsible for the increase of the thermal stability of the Es-tethered tandem duplex in comparison with the natural counterpart.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

The NMR structure of estrone (Es)-tethered tandem DNA duplex: [d(5'pCAGCp3')-Es] + [Es-d(5'pTCCA3')]: d(5'pTGGAGCTG3')., Denisov AY, Sandstrom A, Maltseva TV, Pyshnyi DV, Ivanova EM, Zarytova VF, Chattopadhyaya J, J Biomol Struct Dyn. 1997 Dec;15(3):499-516. PMID:9439997 Page seeded by OCA on Sat May 3 09:44:35 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1tan.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1tan", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tan OCA], [http://www.ebi.ac.uk/pdbsum/1tan PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tan RCSB]</span>
-}}
 '''TANDEM DNA, NMR, MINIMIZED AVERAGE STRUCTURE'''
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 ==About this Structure==
-TAN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAN OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAN OCA].
 ==Reference==
 The NMR structure of estrone (Es)-tethered tandem DNA duplex: [d(5'pCAGCp3')-Es] + [Es-d(5'pTCCA3')]: d(5'pTGGAGCTG3')., Denisov AY, Sandstrom A, Maltseva TV, Pyshnyi DV, Ivanova EM, Zarytova VF, Chattopadhyaya J, J Biomol Struct Dyn. 1997 Dec;15(3):499-516. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9439997 9439997]
-[[Category: Protein complex]]
 [[Category: Chattopadhyaya, J.]]
 [[Category: Denisov, A.]]
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 [[Category: Sandstrom, A.]]
 [[Category: Zarytova, V.]]
-[[Category: deoxyribonucleic acid]]
+[[Category: Deoxyribonucleic acid]]
-[[Category: dna]]
+[[Category: Dna]]
-[[Category: nmr]]
+[[Category: Nmr]]
-[[Category: tandem]]
+[[Category: Tandem]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:44:35 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:53:40 2008''