1tab: Difference between revisions

Revision as of 09:44, 3 May 2008

STRUCTURE OF THE TRYPSIN-BINDING DOMAIN OF BOWMAN-BIRK TYPE PROTEASE INHIBITOR AND ITS INTERACTION WITH TRYPSIN

OverviewOverview

The crystal structure of the complex formed by bovine trypsin and Bowman-Birk type protease inhibitor AB-I extracted from azuki beans (Vigna angularis) 'Takara' has been analyzed. The structure was solved by the application of the phase combination of single isomorphous phases and trypsin model phases, followed by phase improvement using the iterative Fourier technique. From the resulting electron density map, a three-dimensional atomic model of the trypsin binding domain of AB-I has been built. The peptide chain at the trypsin reactive site turns back sharply at Pro29 and forms a 9-residue ring (Cys24-Cys32). The 'front side' of this ring, consisting of the reactive site (Cys24-Met28), interacts with trypsin in a similar manner to other families of inhibitors and forms a stable complex, which seems to be maintained by the interactions with the 'back side' of this ring (Pro29-Cys34). The similar spatial arrangements of the 'back side' of this inhibitor and the 'secondary contact region' of the other inhibitors with respect to the reactive site suggest an important common role of these regions in exhibiting inhibitory activity.

About this StructureAbout this Structure

1TAB is a Protein complex structure. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the trypsin-binding domain of Bowman-Birk type protease inhibitor and its interaction with trypsin., Tsunogae Y, Tanaka I, Yamane T, Kikkawa J, Ashida T, Ishikawa C, Watanabe K, Nakamura S, Takahashi K, J Biochem. 1986 Dec;100(6):1637-46. PMID:3032921 Page seeded by OCA on Sat May 3 09:44:06 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1tab.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1tab |SIZE=350|CAPTION= <scene name='initialview01'>1tab</scene>, resolution 2.3&Aring;
+The line below this paragraph, containing "STRUCTURE_1tab", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1tab|  PDB=1tab  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tab OCA], [http://www.ebi.ac.uk/pdbsum/1tab PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tab RCSB]</span>
-}}
 '''STRUCTURE OF THE TRYPSIN-BINDING DOMAIN OF BOWMAN-BIRK TYPE PROTEASE INHIBITOR AND ITS INTERACTION WITH TRYPSIN'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-TAB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAB OCA].
+TAB is a [[Protein complex]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAB OCA].
 ==Reference==
@@ Line 34: / Line 31: @@
 [[Category: Watanabe, K.]]
 [[Category: Yamane, T.]]
-[[Category: hydrolase (serine proteinase)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:44:06 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:53:31 2008''