Exonuclease: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older editNewer edit →
Michal Harel (talk | contribs)
31,761 edits
No edit summary
Michal Harel (talk | contribs)
31,761 edits
No edit summary
Line 10: Line 10:
* '''ExN-V''' cleaves linear double-stranded DNA.<br />
* '''ExN-V''' cleaves linear double-stranded DNA.<br />
* '''ExN-VIII''' requires a free 5’ OH group for reacting.<br />
* '''ExN-VIII''' requires a free 5’ OH group for reacting.<br />
* '''ExNX''' can degrade both single-stranded DNA and duplex DNA with 3'-5' polarity<ref>PMID:10514496</ref>
* '''ExN-X''' can degrade both single-stranded DNA and duplex DNA with 3'-5' polarity<ref>PMID:10514496</ref>
* '''Trex ExN''' degrades single-stranded DNA generated from processing of aberrant replication intermediates<ref>PMID:18045533</ref>
 
* For '''phage ExN''' see [[Sand box 211]].
* For '''phage ExN''' see [[Sand box 211]].


Revision as of 12:51, 20 June 2024


Exonucleases (ExN) cleave nucleotides at the end of a polynucleotide chain[1]. In E. coli there are ExN I to VIII. ExN were found in human and yeast as well.


  • ExN-I cleaves single-stranded DNA.
  • ExN-II is associated with DNA polymerase.
  • ExN-III cleaves double-stranded DNA.
  • ExN-IV cleavage results in nucleoside monophosphate.
  • ExN-V cleaves linear double-stranded DNA.
  • ExN-VIII requires a free 5’ OH group for reacting.
  • ExN-X can degrade both single-stranded DNA and duplex DNA with 3'-5' polarity[2]
  • Trex ExN degrades single-stranded DNA generated from processing of aberrant replication intermediates[3]

in E. coli exonuclease I (PDB code 1fxx).[4] Water molecules shown as red spheres.

3D Structures of exonuclease

Exonuclease 3D structures


E. coli exonuclease I complex with phosphate, glycerol and Mg+2 ion (PDB code 1fxx)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Mukherjee D, Fritz DT, Kilpatrick WJ, Gao M, Wilusz J. Analysis of RNA exonucleolytic activities in cellular extracts. Methods Mol Biol. 2004;257:193-212. PMID:14770007 doi:http://dx.doi.org/10.1385/1-59259-750-5:193
  2. ↑ Viswanathan M, Lovett ST. Exonuclease X of Escherichia coli. A novel 3'-5' DNase and Dnaq superfamily member involved in DNA repair. J Biol Chem. 1999 Oct 15;274(42):30094-100. PMID:10514496 doi:10.1074/jbc.274.42.30094
  3. ↑ Yang YG, Lindahl T, Barnes DE. Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease. Cell. 2007 Nov 30;131(5):873-86. doi: 10.1016/j.cell.2007.10.017. PMID:18045533 doi:http://dx.doi.org/10.1016/j.cell.2007.10.017
  4. ↑ Breyer WA, Matthews BW. Structure of Escherichia coli exonuclease I suggests how processivity is achieved. Nat Struct Biol. 2000 Dec;7(12):1125-8. PMID:11101894 doi:10.1038/81978

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=Exonuclease&oldid=4212480"