Exonuclease: Difference between revisions
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* '''ExN-V''' cleaves linear double-stranded DNA.<br /> | * '''ExN-V''' cleaves linear double-stranded DNA.<br /> | ||
* '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> | * '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> | ||
* '''ExNX''' can degrade both single-stranded DNA and duplex DNA with 3'-5' polarity<ref>PMID:10514496</ref> | |||
* For '''phage ExN''' see [[Sand box 211]]. | * For '''phage ExN''' see [[Sand box 211]]. | ||
Revision as of 12:40, 20 June 2024
Exonucleases (ExN) cleave nucleotides at the end of a polynucleotide chain[1]. In E. coli there are ExN I to VIII. ExN were found in human and yeast as well.
in E. coli exonuclease I (PDB code 1fxx).[3] Water molecules shown as red spheres. 3D Structures of exonuclease
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ReferencesReferences
- ↑ Mukherjee D, Fritz DT, Kilpatrick WJ, Gao M, Wilusz J. Analysis of RNA exonucleolytic activities in cellular extracts. Methods Mol Biol. 2004;257:193-212. PMID:14770007 doi:http://dx.doi.org/10.1385/1-59259-750-5:193
- ↑ Viswanathan M, Lovett ST. Exonuclease X of Escherichia coli. A novel 3'-5' DNase and Dnaq superfamily member involved in DNA repair. J Biol Chem. 1999 Oct 15;274(42):30094-100. PMID:10514496 doi:10.1074/jbc.274.42.30094
- ↑ Breyer WA, Matthews BW. Structure of Escherichia coli exonuclease I suggests how processivity is achieved. Nat Struct Biol. 2000 Dec;7(12):1125-8. PMID:11101894 doi:10.1038/81978