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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN] | [https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN] | ||
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== Publication Abstract from PubMed == | |||
A ternary complex of the black-eyed pea trypsin and chymotrypsin inhibitor (BTCI) with trypsin and chymotrypsin was crystallized by the sitting-drop vapour-diffusion method with 0.1 M HEPES pH 7.5, 10%(w/v) polyethylene glycol 6000 and 5%(v/v) 2-methyl-2,4-pentanediol as precipitant. BTCI is a small protein with 83 amino-acid residues isolated from Vigna unguiculata seeds and is able to inhibit trypsin and chymotrypsin simultaneously by forming a stable ternary complex. X-ray data were collected from a single crystal of the trypsin-BTCI-chymotrypsin ternary complex to 2.7 A resolution under cryogenic conditions. The structure of the ternary complex was solved by molecular replacement using the crystal structures of the BTCI-trypsin binary complex (PDB code 2g81) and chymotrypsin (PDB code 4cha) as search models. | |||
Crystallization, data collection and processing of the chymotrypsin-BTCI-trypsin ternary complex.,Esteves GF, Teles RC, Cavalcante NS, Neves D, Ventura MM, Barbosa JA, de Freitas SM Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt 12):1087-90., Epub 2007 Nov 30. PMID:018084102<ref>PMID:018084102</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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==See Also== | ==See Also== | ||
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*[[Trypsin 3D structures|Trypsin 3D structures]] | *[[Trypsin 3D structures|Trypsin 3D structures]] | ||
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] | *[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||