3rif: Difference between revisions
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==C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab, ivermectin and glutamate.== | ==C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab, ivermectin and glutamate.== | ||
<StructureSection load='3rif' size='340' side='right'caption='[[3rif]], [[Resolution|resolution]] 3. | <StructureSection load='3rif' size='340' side='right'caption='[[3rif]], [[Resolution|resolution]] 3.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3rif]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The November 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glutamate-gated Chloride Receptors'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_11 10.2210/rcsb_pdb/mom_2015_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIF FirstGlance]. <br> | <table><tr><td colspan='2'>[[3rif]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The November 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glutamate-gated Chloride Receptors'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_11 10.2210/rcsb_pdb/mom_2015_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIF FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rif OCA], [https://pdbe.org/3rif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rif RCSB], [https://www.ebi.ac.uk/pdbsum/3rif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rif ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rif OCA], [https://pdbe.org/3rif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rif RCSB], [https://www.ebi.ac.uk/pdbsum/3rif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rif ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fast inhibitory neurotransmission is essential for nervous system function and is mediated by binding of inhibitory neurotransmitters to receptors of the Cys-loop family embedded in the membranes of neurons. Neurotransmitter binding triggers a conformational change in the receptor, opening an intrinsic chloride channel and thereby dampening neuronal excitability. Here we present the first three-dimensional structure, to our knowledge, of an inhibitory anion-selective Cys-loop receptor, the homopentameric Caenorhabditis elegans glutamate-gated chloride channel alpha (GluCl), at 3.3 A resolution. The X-ray structure of the GluCl-Fab complex was determined with the allosteric agonist ivermectin and in additional structures with the endogenous neurotransmitter l-glutamate and the open-channel blocker picrotoxin. Ivermectin, used to treat river blindness, binds in the transmembrane domain of the receptor and stabilizes an open-pore conformation. Glutamate binds in the classical agonist site at subunit interfaces, and picrotoxin directly occludes the pore near its cytosolic base. GluCl provides a framework for understanding mechanisms of fast inhibitory neurotransmission and allosteric modulation of Cys-loop receptors. | |||
Principles of activation and permeation in an anion-selective Cys-loop receptor.,Hibbs RE, Gouaux E Nature. 2011 May 15. PMID:21572436<ref>PMID:21572436</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3rif" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Ion channels 3D structures|Ion channels 3D structures]] | *[[Ion channels 3D structures|Ion channels 3D structures]] | ||
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||