Enolase: Difference between revisions
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<StructureSection load='1one' size='350' side='right' caption='Yeast enolase dimer complex with phosphoenolpyruvate and phosphoglycerate, [[1one]]' scene='Enolase/Enolase/1'><scene name='Cory_Tiedeman_Sandbox_1/Enolase/1'>Enolase</scene> is an enzyme that catalyzes a reaction of glycolysis. [[Glycolysis]] converts glucose into two 3-carbon molecules called pyruvate. The energy released during glycolysis is used to make ATP.<ref>{{textbook |author=Voet, Donald; Voet, Judith C.; Pratt, Charlotte W.|title=Fundamentals of Biochemistry: Life at the Molecular Level|edition= 3|pages=487|}}</ref> Enolase is used to convert 2-phosphoglycerate (2PG) to phosphoenolpyruvate (PEP) in the 9th reaction of glycolysis: it is a reversible dehydration reaction.<ref>{{textbook |author=Voet, Donald; Voet, Judith C.; Pratt, Charlotte W.|title=Fundamentals of Biochemistry: Life at the Molecular Level|edition= 3|pages=500|}}</ref>. Enolase is expressed abundantly in most cells and has been proven useful as a model to study mechanisms of enzyme action and structural analysis <ref>{{journal}}</ref>. As with the reaction below, Enolase must have a divalent metal cation present to activate or deactivate the enzyme. The best cofactor would be Mg2+, but many, including Zn2+, Mn2+ and Co2+ can be used. The metal ion works by binding to the enzyme at the active site and producing a conformational change. This makes it possible for the substrate (2-PGA) to bind at the Enolase active site. Once this happens, a second metal ion comes in and binds to the enzyme to activate the enolase catalytic ability. See [[Glycolysis Enzymes]]. For sequence alignment see [[Enolase multiple sequence alignment]]. | <StructureSection load='1one' size='350' side='right' caption='Yeast enolase dimer complex with phosphoenolpyruvate and phosphoglycerate, [[1one]]' scene='Enolase/Enolase/1'><scene name='Cory_Tiedeman_Sandbox_1/Enolase/1'>Enolase</scene> is an enzyme that catalyzes a reaction of glycolysis. [[Glycolysis]] converts glucose into two 3-carbon molecules called pyruvate. The energy released during glycolysis is used to make ATP.<ref>{{textbook |author=Voet, Donald; Voet, Judith C.; Pratt, Charlotte W.|title=Fundamentals of Biochemistry: Life at the Molecular Level|edition= 3|pages=487|}}</ref> Enolase is used to convert 2-phosphoglycerate (2PG) to phosphoenolpyruvate (PEP) in the 9th reaction of glycolysis: it is a reversible dehydration reaction.<ref>{{textbook |author=Voet, Donald; Voet, Judith C.; Pratt, Charlotte W.|title=Fundamentals of Biochemistry: Life at the Molecular Level|edition= 3|pages=500|}}</ref>. Enolase is expressed abundantly in most cells and has been proven useful as a model to study mechanisms of enzyme action and structural analysis <ref>{{journal}}</ref>. As with the reaction below, Enolase must have a divalent metal cation present to activate or deactivate the enzyme. The best cofactor would be Mg2+, but many, including Zn2+, Mn2+ and Co2+ can be used. The metal ion works by binding to the enzyme at the active site and producing a conformational change. This makes it possible for the substrate (2-PGA) to bind at the Enolase active site. Once this happens, a second metal ion comes in and binds to the enzyme to activate the enolase catalytic ability. See [[Glycolysis Enzymes]]. For sequence alignment see [[Enolase multiple sequence alignment]]. | ||
*'''Enolase 2''' or '''gamma enolase''' is found in neurons. | |||
*'''2,3-diketo-5-methylthiopentyl-1-phosphate enolase''' is part of the Met salvage pathway. | *'''2,3-diketo-5-methylthiopentyl-1-phosphate enolase''' is part of the Met salvage pathway. | ||