3is2: Difference between revisions
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<StructureSection load='3is2' size='340' side='right'caption='[[3is2]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='3is2' size='340' side='right'caption='[[3is2]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3is2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3is2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IS2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3is2 OCA], [https://pdbe.org/3is2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3is2 RCSB], [https://www.ebi.ac.uk/pdbsum/3is2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3is2 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3is2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3is2 OCA], [https://pdbe.org/3is2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3is2 RCSB], [https://www.ebi.ac.uk/pdbsum/3is2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3is2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9C3Y6_NEUCS Q9C3Y6_NEUCS] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/3is2_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/3is2_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Neurospora crassa]] | |||
[[Category: Phototropin]] | [[Category: Phototropin]] | ||
[[Category: RCSB PDB Molecule of the Month]] | [[Category: RCSB PDB Molecule of the Month]] | ||
[[Category: Crane | [[Category: Crane BR]] | ||
[[Category: Lamb | [[Category: Lamb JS]] | ||
[[Category: Li | [[Category: Li L]] | ||
[[Category: Pabit | [[Category: Pabit SA]] | ||
[[Category: Pollack | [[Category: Pollack L]] | ||
[[Category: Zoltowski | [[Category: Zoltowski BD]] | ||
Latest revision as of 08:54, 17 October 2024
2.3 Angstrom Crystal Structure of a Cys71 Sulfenic Acid form of Vivid2.3 Angstrom Crystal Structure of a Cys71 Sulfenic Acid form of Vivid
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe PAS-LOV domain is a signal-transducing component found in a large variety of proteins that is responsible for sensing different stimuli such as light, oxygen, and voltage. The LOV protein VVD regulates blue light responses in the filamentous fungi Neurospora crassa. Using photocoupled, time-resolved small-angle X-ray scattering, we extract the solution protein structure in both dark-adapted and light-activated states. Two distinct dark-adapted conformations are detected in the wild-type protein: a compact structure that corresponds to the crystal structure of the dark-state monomer as well as an extended structure that is well modeled by introducing conformational disorder at the N-terminus of the protein. These conformations are accentuated in carefully selected variants, in which a key residue for propagating structural transitions, Cys71, has been mutated or oxidized. Despite different dark-state conformations, all proteins form a common dimer in response to illumination. Taken together, these data support a reaction scheme that describes the mechanism for light-induced dimerization of VVD. Envelope reconstructions of the transient light-state dimer reveal structures that are best described by a parallel arrangement of subunits that have significantly changed conformation compared to the crystal structure. Illuminating solution responses of a LOV domain protein with photocoupled small-angle X-ray scattering.,Lamb JS, Zoltowski BD, Pabit SA, Li L, Crane BR, Pollack L J Mol Biol. 2009 Nov 6;393(4):909-19. Epub 2009 Aug 25. PMID:19712683[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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