2gwr: Difference between revisions
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<StructureSection load='2gwr' size='340' side='right'caption='[[2gwr]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='2gwr' size='340' side='right'caption='[[2gwr]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2gwr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2gwr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GWR FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
< | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gwr OCA], [https://pdbe.org/2gwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gwr RCSB], [https://www.ebi.ac.uk/pdbsum/2gwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gwr ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gwr OCA], [https://pdbe.org/2gwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gwr RCSB], [https://www.ebi.ac.uk/pdbsum/2gwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gwr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/MTRA_MYCTU MTRA_MYCTU] Member of the two-component regulatory system MtrA/MtrB. Binds direct repeat motifs of sequence 5'-GTCACAGCG-3', phosphorylation confers higher affinity. Overexpression decreases bacteria viability upon infection of human THP-1 macrophage cell line, due at least in part to impaired blockage of phagosome-lysosome fusion (upon infection bacteria usually remain in phagosomes). Infecting C57BL/6 mice with an overexpressing strain leads to an attentuated infection in both spleen and lungs. The level of dnaA mRNA increases dramatically. Binds the promoter of dnaA, fbpD, ripA and itself, as well as oriC, which it may regulate. Upon co-overexpression of MrtA and MtrB growth in macrophages is partially restored, dnaA expression is not induced, although mouse infections are still attenuated, suggesting that bacterial growth in macrophages requires an optimal ratio of MtrB to MtrA.<ref>PMID:20223818</ref> <ref>PMID:21295603</ref> <ref>PMID:22610443</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/2gwr_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/2gwr_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bursey | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Friedland | [[Category: Bursey EH]] | ||
[[Category: Hung | [[Category: Friedland N]] | ||
[[Category: Mack | [[Category: Hung LW]] | ||
[[Category: Stock | [[Category: Mack TR]] | ||
[[Category: Terwilliger | [[Category: Stock AM]] | ||
[[Category: Waldo | [[Category: Terwilliger TC]] | ||
[[Category: Yu | [[Category: Waldo GS]] | ||
[[Category: Yu M]] | |||