7ki6: Difference between revisions
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==== | ==Structure of the HeV F glycoprotein in complex with the 1F5 neutralizing antibody== | ||
<StructureSection load='7ki6' size='340' side='right'caption='[[7ki6]]' scene=''> | <StructureSection load='7ki6' size='340' side='right'caption='[[7ki6]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ki6]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Henipavirus_hendraense Henipavirus hendraense] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KI6 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ki6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ki6 OCA], [https://pdbe.org/7ki6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ki6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ki6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ki6 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ki6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ki6 OCA], [https://pdbe.org/7ki6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ki6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ki6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ki6 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses (HNVs) causing respiratory illness and severe encephalitis in humans, with fatality rates of 50-100%. There are no licensed therapeutics or vaccines to protect humans. HeV and NiV use a receptor-binding glycoprotein (G) and a fusion glycoprotein (F) to enter host cells. HNV F and G are the main targets of the humoral immune response, and the presence of neutralizing antibodies is a correlate of protection against NiV and HeV in experimentally infected animals. We describe here two cross-reactive F-specific antibodies, 1F5 and 12B2, that neutralize NiV and HeV through inhibition of membrane fusion. Cryo-electron microscopy structures reveal that 1F5 and 12B2 recognize distinct prefusion-specific, conserved quaternary epitopes and lock F in its prefusion conformation. We provide proof-of-concept for using antibody cocktails for neutralizing NiV and HeV and define a roadmap for developing effective countermeasures against these highly pathogenic viruses. | |||
Broadly neutralizing antibody cocktails targeting Nipah virus and Hendra virus fusion glycoproteins.,Dang HV, Cross RW, Borisevich V, Bornholdt ZA, West BR, Chan YP, Mire CE, Da Silva SC, Dimitrov AS, Yan L, Amaya M, Navaratnarajah CK, Zeitlin L, Geisbert TW, Broder CC, Veesler D Nat Struct Mol Biol. 2021 May;28(5):426-434. doi: 10.1038/s41594-021-00584-8. , Epub 2021 Apr 29. PMID:33927387<ref>PMID:33927387</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ki6" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Antibody 3D structures|Antibody 3D structures]] | |||
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Henipavirus hendraense]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mus musculus]] | ||
[[Category: Dang HV]] | |||
[[Category: Veesler D]] | |||