1srn: Difference between revisions

Revision as of 09:03, 3 May 2008

THE REFINED CRYSTAL STRUCTURE OF A FULLY ACTIVE SEMISYNTHETIC RIBONUCLEASE AT 1.8 ANGSTROMS RESOLUTION

OverviewOverview

A fully active, semisynthetic analog of bovine ribonuclease A, comprised of residues 1-118 of the molecule in a noncovalent complex with the synthetic peptide analog of residues 111-124, has been crystallized in space group P3(2)21 from a solution of 1.3 M ammonium sulfate and 3.0 M cesium chloride at pH 5.2. The crystallographic structure was determined by rotation and translation searches utilizing the coordinates for ribonuclease A reported by Wlodawer and Sjolin (Wlodawer, A., and Sjolin, L. (1983) Biochemistry 22, 2720-2728) and has been refined at 1.8-A resolution to an agreement factor of 0.204. Most of the structure of the semisynthetic enzyme closely resembles that found in ribonuclease A with the synthetic peptide replacing the C-terminal elements of the naturally occurring enzyme. No redundant structure is seen; residues 114-118 of the larger chain and residues 111-113 of the peptide do not appear in our map. The positions of those residues at or near the active site are very similar to, if not identical with, those previously reported by others, except for histidine 119, which occupies predominantly the B position seen as a minor site by Borkakoti et al. (Borkakoti, N., Moss, D. S., and Palmer, R. A. (1982) Acta Crystallogr. Sect. B Struct. Crystallogr. Cryst. Chem. 38,2210-2217) and not at all by Wlodawer and Sjolin (1983).

About this StructureAbout this Structure

1SRN is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

The refined crystal structure of a fully active semisynthetic ribonuclease at 1.8-A resolution., Martin PD, Doscher MS, Edwards BF, J Biol Chem. 1987 Nov 25;262(33):15930-8. PMID:3680234 Page seeded by OCA on Sat May 3 09:03:54 2008

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OCA

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 [[Image:1srn.gif|left|200px]]
- {{Structure
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-|PDB= 1srn |SIZE=350|CAPTION= <scene name='initialview01'>1srn</scene>, resolution 1.8&Aring;
+The line below this paragraph, containing "STRUCTURE_1srn", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1srn|  PDB=1srn  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1srn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1srn OCA], [http://www.ebi.ac.uk/pdbsum/1srn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1srn RCSB]</span>
-}}
 '''THE REFINED CRYSTAL STRUCTURE OF A FULLY ACTIVE SEMISYNTHETIC RIBONUCLEASE AT 1.8 ANGSTROMS RESOLUTION'''
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 [[Category: Edwards, B F.P.]]
 [[Category: Martin, P D.]]
-[[Category: hydrolase (nucleic acid,rna)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:03:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:46:04 2008''