1sos: Difference between revisions

Revision as of 08:57, 3 May 2008

ATOMIC STRUCTURES OF WILD-TYPE AND THERMOSTABLE MUTANT RECOMBINANT HUMAN CU, ZN SUPEROXIDE DISMUTASE

OverviewOverview

Superoxide dismutase enzymes protect aerobic organisms from oxygen-mediated free-radical damage. Crystallographic structures of recombinant human Cu,Zn superoxide dismutase have been determined, refined, and analyzed at 2.5 A resolution for wild-type and a designed thermostable double-mutant enzyme (Cys-6----Ala, Cys-111----Ser). The 10 subunits (five dimers) in the crystallographic asymmetric unit form an unusual stable open lattice with 80-A-diameter channels. The 10 independently fit and refined subunits provide high accuracy, error analysis, and insights on loop conformations. There is a helix dipole interaction with the Zn site, and 14 residues form two or more structurally conserved side-chain to main-chain hydrogen bonds that appear critical to active-site architecture, loop conformation, and the increased stability resulting from the Cys-111----Ser mutation.

About this StructureAbout this Structure

1SOS is a Single protein structure of sequence from Homo sapiens. The following page contains interesting information on the relation of 1SOS with [Superoxide Dismutase]. Full crystallographic information is available from OCA.

ReferenceReference

Atomic structures of wild-type and thermostable mutant recombinant human Cu,Zn superoxide dismutase., Parge HE, Hallewell RA, Tainer JA, Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6109-13. PMID:1463506 Page seeded by OCA on Sat May 3 08:57:43 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1sos.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1sos |SIZE=350|CAPTION= <scene name='initialview01'>1sos</scene>, resolution 2.5&Aring;
+The line below this paragraph, containing "STRUCTURE_1sos", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1sos|  PDB=1sos  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sos OCA], [http://www.ebi.ac.uk/pdbsum/1sos PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sos RCSB]</span>
-}}
 '''ATOMIC STRUCTURES OF WILD-TYPE AND THERMOSTABLE MUTANT RECOMBINANT HUMAN CU, ZN SUPEROXIDE DISMUTASE'''
@@ Line 30: / Line 27: @@
 [[Category: Parge, H E.]]
 [[Category: Tainer, J A.]]
-[[Category: oxidoreductase (superoxide acceptor)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:57:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:45:00 2008''