1smh: Difference between revisions

Revision as of 08:53, 3 May 2008

Protein kinase A variant complex with completely ordered N-terminal helix

OverviewOverview

Protein kinases comprise the major enzyme family critically involved in signal transduction pathways; posttranslational modifications affect their regulation and determine signaling states. The prototype protein kinase A (PKA) possesses an N-terminal alpha-helix (Helix A) that is atypical for kinases and is thus a major distinguishing feature of PKA. Its physiological function may involve myristoylation at the N-terminus and modulation via phosphorylation at serine 10. Here we describe an unusual structure of an unmyristoylated PKA, unphosphorylated at serine 10, with a completely ordered N-terminus. Using standard conditions (e.g., PKI 5-24, ATP site ligand, MEGA-8), a novel 2-fold phosphorylated PKA variant showed the ordered N-terminus in a new crystal packing arrangement. Thus, the critical factor for structuring the N-terminus is apparently the absence of phosphorylation of Ser10. The flexibility of the N-terminus, its myristoylation, and the conformational dependence on the phosphorylation state are consistent with a functional role for myristoylation.

About this StructureAbout this Structure

1SMH is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

The typically disordered N-terminus of PKA can fold as a helix and project the myristoylation site into solution., Breitenlechner C, Engh RA, Huber R, Kinzel V, Bossemeyer D, Gassel M, Biochemistry. 2004 Jun 22;43(24):7743-9. PMID:15196017 Page seeded by OCA on Sat May 3 08:53:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1smh.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1smh |SIZE=350|CAPTION= <scene name='initialview01'>1smh</scene>, resolution 2.044&Aring;
+The line below this paragraph, containing "STRUCTURE_1smh", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BU3:(R,R)-2,3-BUTANEDIOL'>BU3</scene>, <scene name='pdbligand=MG8:N-OCTANOYL-N-METHYLGLUCAMINE'>MG8</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PRKACA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
+-->
-|DOMAIN=
+{{STRUCTURE_1smh|  PDB=1smh  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1smh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1smh OCA], [http://www.ebi.ac.uk/pdbsum/1smh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1smh RCSB]</span>
-}}
 '''Protein kinase A variant complex with completely ordered N-terminal helix'''
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 [[Category: Huber, R.]]
 [[Category: Kinzel, V.]]
-[[Category: alpha helix]]
+[[Category: Alpha helix]]
-[[Category: camp-dependent protein kinase]]
+[[Category: Camp-dependent protein kinase]]
-[[Category: membrane]]
+[[Category: Membrane]]
-[[Category: myristoylation]]
+[[Category: Myristoylation]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: pka]]
+[[Category: Pka]]
-[[Category: posttranslational modification]]
+[[Category: Posttranslational modification]]
-[[Category: protein kinase some]]
+[[Category: Protein kinase some]]
-[[Category: ser10]]
+[[Category: Ser10]]
-[[Category: signaling]]
+[[Category: Signaling]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:53:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:44:06 2008''