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==Crystal Structure of H+/Ca2+ Exchanger CAX== | ==Crystal Structure of H+/Ca2+ Exchanger CAX== | ||
<StructureSection load='4kpp' size='340' side='right'caption='[[4kpp]]' scene=''> | <StructureSection load='4kpp' size='340' side='right'caption='[[4kpp]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KPP FirstGlance]. <br> | <table><tr><td colspan='2'>[[4kpp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KPP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpp OCA], [https://pdbe.org/4kpp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kpp RCSB], [https://www.ebi.ac.uk/pdbsum/4kpp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpp ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpp OCA], [https://pdbe.org/4kpp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kpp RCSB], [https://www.ebi.ac.uk/pdbsum/4kpp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpp ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/O29988_ARCFU O29988_ARCFU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ca2+/cation antiporters catalyze the exchange of Ca2+ with various cations across biological membranes, to regulate cytosolic calcium levels. The recently reported structure of a prokaryotic Na+/Ca2+ exchanger (NCX_Mj) revealed its overall architecture in an outward-facing state. Here we report the crystal structure of a H+/Ca2+ exchanger from Archaeoglobus fulgidus (CAX_Af) in the two representatives of the inward-facing conformation at 2.3 A resolution. The structures suggested Ca2+ or H+ binds to the cation-binding site mutually exclusively. Structural comparison of CAX_Af with NCX_Mj revealed that the first and sixth transmembrane helices alternately create hydrophilic cavities on the intra- and extracellular sides. The structures and functional analyses provide insight into the mechanism of how the inward- to outward-facing state transition is triggered by the Ca2+ and H+ binding. | |||
Structural Basis for the Counter-Transport Mechanism of a H+/Ca2+ Exchanger.,Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O Science. 2013 May 23. PMID:23704374<ref>PMID:23704374</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4kpp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Archaeoglobus fulgidus DSM 4304]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ishitani R]] | [[Category: Ishitani R]] | ||
[[Category: Nishizawa T]] | [[Category: Nishizawa T]] | ||
[[Category: Nureki O]] | [[Category: Nureki O]] | ||
Latest revision as of 22:23, 29 May 2024
Crystal Structure of H+/Ca2+ Exchanger CAXCrystal Structure of H+/Ca2+ Exchanger CAX
Structural highlights
FunctionPublication Abstract from PubMedCa2+/cation antiporters catalyze the exchange of Ca2+ with various cations across biological membranes, to regulate cytosolic calcium levels. The recently reported structure of a prokaryotic Na+/Ca2+ exchanger (NCX_Mj) revealed its overall architecture in an outward-facing state. Here we report the crystal structure of a H+/Ca2+ exchanger from Archaeoglobus fulgidus (CAX_Af) in the two representatives of the inward-facing conformation at 2.3 A resolution. The structures suggested Ca2+ or H+ binds to the cation-binding site mutually exclusively. Structural comparison of CAX_Af with NCX_Mj revealed that the first and sixth transmembrane helices alternately create hydrophilic cavities on the intra- and extracellular sides. The structures and functional analyses provide insight into the mechanism of how the inward- to outward-facing state transition is triggered by the Ca2+ and H+ binding. Structural Basis for the Counter-Transport Mechanism of a H+/Ca2+ Exchanger.,Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O Science. 2013 May 23. PMID:23704374[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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