2jxb: Difference between revisions
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==Structure of CD3epsilon-Nck2 first SH3 domain complex== | ==Structure of CD3epsilon-Nck2 first SH3 domain complex== | ||
<StructureSection load='2jxb' size='340' side='right'caption='[[2jxb | <StructureSection load='2jxb' size='340' side='right'caption='[[2jxb]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2jxb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JXB FirstGlance]. <br> | <table><tr><td colspan='2'>[[2jxb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JXB FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxb OCA], [https://pdbe.org/2jxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jxb RCSB], [https://www.ebi.ac.uk/pdbsum/2jxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxb ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxb OCA], [https://pdbe.org/2jxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jxb RCSB], [https://www.ebi.ac.uk/pdbsum/2jxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/NCK2_HUMAN NCK2_HUMAN] Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.<ref>PMID:10026169</ref> <ref>PMID:16835242</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ng | [[Category: Ng E]] | ||
[[Category: Park | [[Category: Park S]] | ||
[[Category: Reinherz | [[Category: Reinherz EL]] | ||
[[Category: Sun | [[Category: Sun ZJ]] | ||
[[Category: Takeuchi | [[Category: Takeuchi K]] | ||
[[Category: Wagner | [[Category: Wagner G]] | ||
[[Category: Yang | [[Category: Yang H]] | ||
Latest revision as of 22:06, 29 May 2024
Structure of CD3epsilon-Nck2 first SH3 domain complexStructure of CD3epsilon-Nck2 first SH3 domain complex
Structural highlights
FunctionNCK2_HUMAN Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedRecruitment of signaling molecules to the cytoplasmic domains of the CD3 subunits of the T-cell receptor (TCR) is crucial for early T-cell activation. These transient associations either do or do not require tyrosine phosphorylation of CD3 immune tyrosine activation motifs (ITAMs). Here we show that the non-ITAM-requiring adaptor protein Nck forms a complex with an atypical PxxDY motif of the CD3epsilon tail, which encompasses Tyr166 within the ITAM and a TCR endocytosis signal. As suggested by the structure of the complex, we find that Nck binding inhibits phosphorylation of the CD3epsilon ITAM by Fyn and Lck kinases in vitro. Moreover, the CD3epsilon-Nck interaction downregulates TCR surface expression upon physiological stimulation in mouse primary lymph node cells. This indicates that Nck performs an important regulatory function in T lymphocytes by inhibiting ITAM phosphorylation and/or removing cell surface TCR via CD3epsilon interaction. Structural and functional evidence that Nck interaction with CD3epsilon regulates T-cell receptor activity.,Takeuchi K, Yang H, Ng E, Park SY, Sun ZY, Reinherz EL, Wagner G J Mol Biol. 2008 Jul 18;380(4):704-16. Epub 2008 May 22. PMID:18555270[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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