1siz: Difference between revisions

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Crystal structure of the [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus

OverviewOverview

The structure of [Fe(3)S(4)]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus has been determined to 1.5 A resolution from a crystal belonging to space group P2(1) with two molecules in the asymmetric unit. The structure has been solved with molecular replacement by use of the ferredoxin from Thermotoga maritima. The fold is similar to that of related monocluster ferredoxins and contains two double-stranded antiparallel beta-sheets and two alpha-helices. The hydrophobic interaction between Trp2 and Tyr46 is confirmed, linking the C-terminus to the longer alpha-helix. The structure contains a double-conformation disulfide bond existing in a left-handed and a right-handed spiral conformation. The crystal packing reveals a beta-sheet interaction, which supports the suggestion that P. furiosus ferredoxin is a functional dimer. The extraordinary thermostability of P. furiosus ferredoxin is further discussed.

About this StructureAbout this Structure

1SIZ is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

ReferenceReference

The 1.5 A resolution crystal structure of [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus., Nielsen MS, Harris P, Ooi BL, Christensen HE, Biochemistry. 2004 May 11;43(18):5188-94. PMID:15122884 Page seeded by OCA on Sat May 3 08:45:29 2008

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OCA

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 '''Crystal structure of the [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus'''
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-[[Category: dimer]]
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-[[Category: iron-sulfur cluster]]
+[[Category: Iron-sulfur cluster]]
-[[Category: thermostability]]
+[[Category: Thermostability]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:45:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:42:38 2008''