1si8: Difference between revisions

Revision as of 08:44, 3 May 2008

Crystal structure of E. faecalis catalase

OverviewOverview

Enterococcus faecalis haem catalase was crystallized using lithium sulfate at neutral pH. The crystals belong to space group R3, with unit-cell parameters a = b = 236.9, c = 198.1 A. The three-dimensional structure was determined by molecular replacement using a subunit of the Proteus mirabilis catalase structure. It was refined against 2.3 A synchrotron data to a free R factor of 21.8%. Like other catalases, the E. faecalis catalase is a homotetramer with a fold and structure similar to those of its structurally closest relative P. mirabilis. The solvent structure in the active site is identical in the four subunits but differs from that found in other catalases. The structural consequences of the Ramachandran outlier Ser196 are discussed.

About this StructureAbout this Structure

1SI8 is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.

ReferenceReference

The three-dimensional structure of catalase from Enterococcus faecalis., Hakansson KO, Brugna M, Tasse L, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1374-80. Epub 2004, Jul 21. PMID:15272159 Page seeded by OCA on Sat May 3 08:44:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1si8.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1si8 |SIZE=350|CAPTION= <scene name='initialview01'>1si8</scene>, resolution 2.30&Aring;
+The line below this paragraph, containing "STRUCTURE_1si8", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= katA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 Enterococcus faecalis])
+-->
-|DOMAIN=
+{{STRUCTURE_1si8|  PDB=1si8  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1si8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1si8 OCA], [http://www.ebi.ac.uk/pdbsum/1si8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1si8 RCSB]</span>
-}}
 '''Crystal structure of E. faecalis catalase'''
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 [[Category: Hakansson, K O.]]
 [[Category: Tasse, L.]]
-[[Category: anti-parallel beta-barrel]]
+[[Category: Anti-parallel beta-barrel]]
-[[Category: c-terminal helical region]]
+[[Category: C-terminal helical region]]
-[[Category: heme group]]
+[[Category: Heme group]]
-[[Category: n-terminal arm]]
+[[Category: N-terminal arm]]
-[[Category: tetramer]]
+[[Category: Tetramer]]
-[[Category: wrapping region]]
+[[Category: Wrapping region]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:44:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:42:28 2008''