1sdo: Difference between revisions

Revision as of 08:35, 3 May 2008

Crystal Structure of Restriction Endonuclease BstYI

OverviewOverview

We report here the structure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence specificities to BamHI and BglII. BstYI, a thermophilic endonuclease, recognizes and cleaves the degenerate hexanucleotide sequence 5'-RGATCY-3' (where R=A or G and Y=C or T), cleaving DNA after the 5'-R on each strand to produce four-base (5') staggered ends. The crystal structure of free BstYI was solved at 1.85A resolution by multi-wavelength anomalous dispersion (MAD) phasing. Comparison with BamHI and BglII reveals a strong structural consensus between all three enzymes mapping to the alpha/beta core domain and residues involved in catalysis. Unexpectedly, BstYI also contains an additional "arm" substructure outside of the core protein, which enables the enzyme to adopt a more compact, intertwined dimer structure compared with BamHI and BglII. This arm substructure may underlie the thermostability of BstYI. We identify putative DNA recognition residues and speculate as to how this enzyme achieves a "relaxed" DNA specificity.

About this StructureAbout this Structure

1SDO is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of BstYI at 1.85A resolution: a thermophilic restriction endonuclease with overlapping specificities to BamHI and BglII., Townson SA, Samuelson JC, Vanamee ES, Edwards TA, Escalante CR, Xu SY, Aggarwal AK, J Mol Biol. 2004 May 7;338(4):725-33. PMID:15099740 Page seeded by OCA on Sat May 3 08:35:01 2008

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OCA

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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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-}}
 '''Crystal Structure of Restriction Endonuclease BstYI'''
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 [[Category: Vanamee, E S.]]
 [[Category: Xu, S Y.]]
-[[Category: restriction endonuclease]]
+[[Category: Restriction endonuclease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:35:01 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:40:40 2008''