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'''CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX''' | '''CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX''' | ||
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[[Category: Berman, H M.]] | [[Category: Berman, H M.]] | ||
[[Category: Jain, S C.]] | [[Category: Jain, S C.]] | ||
[[Category: | [[Category: Hydrolase]] | ||
[[Category: | [[Category: Serine protease]] | ||
[[Category: | [[Category: Subtilisin e - propeptide]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:32:50 2008'' | |||
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Revision as of 08:32, 3 May 2008
CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX
OverviewOverview
We report here the crystallographic structure determination of an autoprocessed (Ser221Cys)-subtilisin E-propeptide complex at 2.0 A resolution. The subtilisin domain sequence has a single substitution (Ser221Cys) which has been shown to block the maturation process prior to degradation of the propeptide domain (77 residues) that acts as an intramolecular chaperon. This mutation, however, did not prevent the enzyme from cleaving its propeptide domain with a 60-80% efficiency. The current determination is the first example of a subtilisin E-propeptide complex which has been autoprocessed. A previous structure determination of a BPN'-prosegment complex has been reported in which the subtilisin domain was extensively mutated and a calcium binding loop was deleted. Further, in this earlier determination, the complex was formed by the addition of separately expressed propeptide domain. The structure determination reported here provides additional information about the nature of the interaction between the subtilisin and propeptide domains in this complex.
About this StructureAbout this Structure
1SCJ is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution., Jain SC, Shinde U, Li Y, Inouye M, Berman HM, J Mol Biol. 1998 Nov 20;284(1):137-44. PMID:9811547 Page seeded by OCA on Sat May 3 08:32:50 2008