1sb7: Difference between revisions

Revision as of 08:30, 3 May 2008

Crystal structure of the E.coli pseudouridine synthase TruD

OverviewOverview

The pseudouridine (Psi) synthases Pus7p and TruD define a family of RNA-modifying enzymes with no sequence similarity to previously characterized Psi synthases. The 2.2 A resolution structure of Escherichia coli TruD reveals a U-shaped molecule with a catalytic domain that superimposes closely on that of other Psi synthases. A domain that appears to be unique to TruD/Pus7p family enzymes hinges over the catalytic domain, possibly serving to clasp the substrate RNAs. The active site comprises residues that are conserved in other Psi synthases, although at least one comes from a structurally distinct part of the protein. Remarkably, the connectivity of the structural elements of the TruD catalytic domain is a circular permutation of that of its paralogs. Because the sequence of the permuted segment, a beta-strand that bisects the catalytic domain, is conserved among orthologs from bacteria, archaea and eukarya, the permutation likely happened early in evolution.

About this StructureAbout this Structure

1SB7 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the highly divergent pseudouridine synthase TruD reveals a circular permutation of a conserved fold., Hoang C, Ferre-D'Amare AR, RNA. 2004 Jul;10(7):1026-33. PMID:15208439 Page seeded by OCA on Sat May 3 08:30:15 2008

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OCA

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- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] </span>
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-|GENE= TRUD, B2745 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-}}
 '''Crystal structure of the E.coli pseudouridine synthase TruD'''
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 [[Category: Amare, A R.Ferre-D.]]
 [[Category: Hoang, C.]]
-[[Category: pseudouridine synthase]]
+[[Category: Pseudouridine synthase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:30:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:45 2008''