1saq: Difference between revisions

Revision as of 08:29, 3 May 2008

Crystal Structure of the RNA octamer GIC(GA)GCC

OverviewOverview

The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Structures of two RNA octamers containing tandem G.A base pairs., Jang SB, Baeyens K, Jeong MS, SantaLucia J Jr, Turner D, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):829-35. Epub 2004, Apr 21. PMID:15103128 Page seeded by OCA on Sat May 3 08:29:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1saq.gif|left|200px]]
- {{Structure
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-}}
 '''Crystal Structure of the RNA octamer GIC(GA)GCC'''
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 ==About this Structure==
-SAQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAQ OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAQ OCA].
 ==Reference==
 Structures of two RNA octamers containing tandem G.A base pairs., Jang SB, Baeyens K, Jeong MS, SantaLucia J Jr, Turner D, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):829-35. Epub 2004, Apr 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15103128 15103128]
-[[Category: Protein complex]]
 [[Category: Baeyens, K.]]
 [[Category: Holbrook, S R.]]
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 [[Category: Jr, J SantaLucia.]]
 [[Category: Turner, D.]]
-[[Category: rna double helix]]
+[[Category: Rna double helix]]
-[[Category: rna tandem g-a base pair]]
+[[Category: Rna tandem g-a base pair]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:29:21 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:31 2008''