1s95: Difference between revisions

Revision as of 08:26, 3 May 2008

Structure of serine/threonine protein phosphatase 5

OverviewOverview

Serine/threonine protein phosphatase-5 (PP5) affects many signaling networks that regulate cell growth and cellular responses to stress. Here we report the crystal structure of the PP5 catalytic domain (PP5c) at a resolution of 1.6 A. From this structure we propose a mechanism for PP5-mediated hydrolysis of phosphoprotein substrates, which requires the precise positioning of two metal ions within a conserved Asp271-M1:M2-W1-His427-His304-Asp274 catalytic motif (where M1 and M2 are metals and W1 is a water molecule). The structure of PP5c provides a structural basis for explaining the exceptional catalytic proficiency of protein phosphatases, which are among the most powerful known catalysts. Resolution of the entire C terminus revealed a novel subdomain, and the structure of the PP5c should also aid development of type-specific inhibitors.

About this StructureAbout this Structure

1S95 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the catalytic activity of human serine/threonine protein phosphatase-5., Swingle MR, Honkanen RE, Ciszak EM, J Biol Chem. 2004 Aug 6;279(32):33992-9. Epub 2004 May 23. PMID:15155720 Page seeded by OCA on Sat May 3 08:26:16 2008

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OCA

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 [[Image:1s95.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1s95 |SIZE=350|CAPTION= <scene name='initialview01'>1s95</scene>, resolution 1.60&Aring;
+The line below this paragraph, containing "STRUCTURE_1s95", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PPP5C, PPP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_1s95|  PDB=1s95  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s95 OCA], [http://www.ebi.ac.uk/pdbsum/1s95 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s95 RCSB]</span>
-}}
 '''Structure of serine/threonine protein phosphatase 5'''
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 [[Category: Honkanen, R E.]]
 [[Category: Swingle, M R.]]
-[[Category: metal ion]]
+[[Category: Metal ion]]
-[[Category: metallophosphoesterase]]
+[[Category: Metallophosphoesterase]]
-[[Category: phosphate anion]]
+[[Category: Phosphate anion]]
-[[Category: pp5]]
+[[Category: Pp5]]
-[[Category: pppase]]
+[[Category: Pppase]]
-[[Category: protein phosphatase]]
+[[Category: Protein phosphatase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:26:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:38:55 2008''