2m1j: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2m1j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M1J FirstGlance]. <br> | <table><tr><td colspan='2'>[[2m1j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M1J FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m1j OCA], [https://pdbe.org/2m1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m1j RCSB], [https://www.ebi.ac.uk/pdbsum/2m1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m1j ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m1j OCA], [https://pdbe.org/2m1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m1j RCSB], [https://www.ebi.ac.uk/pdbsum/2m1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m1j ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
Latest revision as of 08:57, 15 May 2024
Ovine Doppel Signal peptide (1-30)Ovine Doppel Signal peptide (1-30)
Structural highlights
FunctionPublication Abstract from PubMedPrion protein (PrPC) biosynthesis involves a multi-step process that includes translation and post-translational modifications. While PrP has been widely investigated, for the homolog Doppel (Dpl), limited knowledge is available. In this study, we focused on a vital step of eukaryotic protein biosynthesis: targeting by the signal recognition particle (SRP). Taking the ovine Dpl (OvDpl(1-30)) peptide as a template, we studied its behavior in two different hydrophobic environments using CD and NMR spectroscopy. In both trifluoroethanol (TFE) and dihexanoyl-sn-glycero-3-phosphatidylcholine (DHPC), the OvDpl(1-30) peptide revealed to fold in an alpha-helical conformation with a well-defined central region extending from residue Cys8 until Ser22. The NMR structure was subsequently included in a computational docking complex with the conserved M-domain of SRP54 protein (SRP54M), and further compared with the N-terminal structures of mouse Dpl and bovine PrPC proteins. This allowed the determination of (i) common predicted N-terminal/SRP54M polar contacts (Asp331, Gln335, Glu365 and Lys432) and (ii) different N-C orientations between prion and Dpl peptides at the SRP54M hydrophobic groove, that are in agreement with each peptide electrostatic potential. Together, these findings provide new insights into the biosynthesis of prion-like proteins. Besides they also show the role of protein conformational switches in signalization toward the endoplasmic membrane, a key event of major significance in the cell cycle. They are thus of general applicability to the study of the biological function of prion-like as well as other proteins. NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein.,Pimenta J, Viegas A, Sardinha J, Martins IC, Cabrita EJ, Fontes CM, Prates JA, Pereira RM Peptides. 2013 Aug 23;49C:32-40. doi: 10.1016/j.peptides.2013.08.013. PMID:23973967[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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