6f4h: Difference between revisions
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==Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complex== | ==Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complex== | ||
<StructureSection load='6f4h' size='340' side='right' caption='[[6f4h]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='6f4h' size='340' side='right'caption='[[6f4h]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6f4h]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F4H OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6f4h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F4H FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f4h OCA], [https://pdbe.org/6f4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f4h RCSB], [https://www.ebi.ac.uk/pdbsum/6f4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f4h ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/SNRPA_DROME SNRPA_DROME] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP (By similarity). Plays a role in regulating sex-lethal splicing. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 6f4h" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6f4h" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Drosophila melanogaster]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: DeKoster G]] | ||
[[Category: Hall KB]] | |||
[[Category: Holton N]] | |||
[[Category: | [[Category: Wahl MC]] | ||
[[Category: | [[Category: Weber G]] | ||
[[Category: | |||
[[Category: | |||
Latest revision as of 15:24, 9 May 2024
Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complexCrystal structure of the Drosophila melanogaster SNF/U1-SL2 complex
Structural highlights
FunctionSNRPA_DROME Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP (By similarity). Plays a role in regulating sex-lethal splicing. Publication Abstract from PubMedThe first RNA recognition motif of the Drosophila SNF protein is an example of an RNA binding protein with multi-specificity. It binds different RNA hairpin loops in spliceosomal U1 or U2 small nuclear RNAs, and only in the latter case requires the auxiliary U2A' protein. Here we investigate its functions by crystal structures of SNF alone and bound to U1 stem-loop II, U2A' or U2 stem-loop IV and U2A', SNF dynamics from NMR spectroscopy, and structure-guided mutagenesis in binding studies. We find that different loop-closing base pairs and a nucleotide exchange at the tips of the loops contribute to differential SNF affinity for the RNAs. U2A' immobilizes SNF and RNA residues to restore U2 stem-loop IV binding affinity, while U1 stem-loop II binding does not require such adjustments. Our findings show how U2A' can modulate RNA specificity of SNF without changing SNF conformation or relying on direct RNA contacts. Molecular principles underlying dual RNA specificity in the Drosophila SNF protein.,Weber G, DeKoster GT, Holton N, Hall KB, Wahl MC Nat Commun. 2018 Jun 7;9(1):2220. doi: 10.1038/s41467-018-04561-6. PMID:29880797[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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