5a1v: Difference between revisions

Latest revision as of 14:35, 9 May 2024

The structure of the COPI coat linkage IThe structure of the COPI coat linkage I

5a1v, resolution 21.00Å

Structural highlights

5a1v is a 26 chain structure with sequence from Mus musculus and Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 21Å
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ARF1_YEAST GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.^[1]

Publication Abstract from PubMed

Transport of material within cells is mediated by trafficking vesicles that bud from one cellular compartment and fuse with another. Formation of a trafficking vesicle is driven by membrane coats that localize cargo and polymerize into cages to bend the membrane. Although extensive structural information is available for components of these coats, the heterogeneity of trafficking vesicles has prevented an understanding of how complete membrane coats assemble on the membrane. We combined cryo-electron tomography, subtomogram averaging, and cross-linking mass spectrometry to derive a complete model of the assembled coat protein complex I (COPI) coat involved in traffic between the Golgi and the endoplasmic reticulum. The highly interconnected COPI coat structure contradicted the current "adaptor-and-cage" understanding of coated vesicle formation.

VESICULAR TRANSPORT. A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly.,Dodonova SO, Diestelkoetter-Bachert P, von Appen A, Hagen WJ, Beck R, Beck M, Wieland F, Briggs JA Science. 2015 Jul 10;349(6244):195-8. doi: 10.1126/science.aab1121. PMID:26160949^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Trautwein M, Dengjel J, Schirle M, Spang A. Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae. Mol Biol Cell. 2004 Nov;15(11):5021-37. Epub 2004 Sep 8. PMID:15356266 doi:http://dx.doi.org/10.1091/mbc.E04-05-0411
↑ Dodonova SO, Diestelkoetter-Bachert P, von Appen A, Hagen WJ, Beck R, Beck M, Wieland F, Briggs JA. VESICULAR TRANSPORT. A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly. Science. 2015 Jul 10;349(6244):195-8. doi: 10.1126/science.aab1121. PMID:26160949 doi:http://dx.doi.org/10.1126/science.aab1121

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OCA

[1] Trautwein M, Dengjel J, Schirle M, Spang A. Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae. Mol Biol Cell. 2004 Nov;15(11):5021-37. Epub 2004 Sep 8. PMID:15356266 doi:http://dx.doi.org/10.1091/mbc.E04-05-0411

[2] Dodonova SO, Diestelkoetter-Bachert P, von Appen A, Hagen WJ, Beck R, Beck M, Wieland F, Briggs JA. VESICULAR TRANSPORT. A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly. Science. 2015 Jul 10;349(6244):195-8. doi: 10.1126/science.aab1121. PMID:26160949 doi:http://dx.doi.org/10.1126/science.aab1121

[1]

[2]

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5a1v]] is a 26 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A1V FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a1v OCA], [https://pdbe.org/5a1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a1v RCSB], [https://www.ebi.ac.uk/pdbsum/5a1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a1v ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 21&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a1v OCA], [https://pdbe.org/5a1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a1v RCSB], [https://www.ebi.ac.uk/pdbsum/5a1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a1v ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 </div>
 <div class="pdbe-citations 5a1v" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Coatomer 3D structures|Coatomer 3D structures]]
 == References ==
 <references/>

5a1v: Difference between revisions

Latest revision as of 14:35, 9 May 2024

The structure of the COPI coat linkage IThe structure of the COPI coat linkage I

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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5a1v: Difference between revisions

Latest revision as of 14:35, 9 May 2024

The structure of the COPI coat linkage IThe structure of the COPI coat linkage I

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

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