4a39: Difference between revisions
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<StructureSection load='4a39' size='340' side='right'caption='[[4a39]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='4a39' size='340' side='right'caption='[[4a39]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4a39]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4a39]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A39 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GEM:(2-GUANIDINOETHYLMERCAPTO)SUCCINIC+ACID'>GEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a39 OCA], [https://pdbe.org/4a39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a39 RCSB], [https://www.ebi.ac.uk/pdbsum/4a39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a39 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a39 OCA], [https://pdbe.org/4a39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a39 RCSB], [https://www.ebi.ac.uk/pdbsum/4a39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a39 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9I012_PSEAE Q9I012_PSEAE] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Aviles | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Lorenzo | [[Category: Aviles FX]] | ||
[[Category: Otero | [[Category: Lorenzo J]] | ||
[[Category: Reverter | [[Category: Otero A]] | ||
[[Category: | [[Category: Reverter D]] | ||
[[Category: Rodriguez de la Vega M]] | |||
[[Category: | [[Category: Tanco SM]] | ||
Latest revision as of 13:46, 9 May 2024
Metallo-carboxypeptidase from Pseudomonas Aeruginosa in complex with (2-guanidinoethylmercapto)succinic acidMetallo-carboxypeptidase from Pseudomonas Aeruginosa in complex with (2-guanidinoethylmercapto)succinic acid
Structural highlights
FunctionPublication Abstract from PubMedPaCCP is a metallocarboxypeptidase (MCP) of the M14 family from Pseudomonas aeruginosa, which belongs to a bacterial clade of carboxypeptidases that are homologous to the recently discovered M14D subfamily of human nonsecretory cytosolic carboxypeptidases (CCPs). CCPs are intracellular peptidases involved, among other roles, in the post-translational modifications of tubulin. Here we report the crystal structure of PaCCP at high resolution (1.6 A). Its 375 residues are folded in a novel beta-sandwich N-terminal domain followed by the classical carboxypeptidase alpha/beta-hydrolase domain, this one in a shorter and more compact form. The former is unique in the whole family and does not have sequential or structural homology with other domains that are usually flanking the latter, like the prodomain of the M14A subfamily or the C-terminal transthyretin/prealbumin-like domains of the M14B subfamily. PaCCP does not display activity against small carboxypeptidase substrates, so in this form it might constitute an inactive precursor of the protease. Structural results derived from cocrystallization with well-known inhibitors of MCPs indicate that the enzyme might only possess C-terminal hydrolase activity against cellular substrates of particular specificity and/or when undergoes structural rearrangements. The derived PaCCP structure allows a first structural insight into the more complex and largely unknown mammalian CCP subfamily.-Otero, A., Rodriguez de la Vega, M., Tanco, S., Lorenzo, J., Aviles, F. X., Reverter, D. The novel structure of a cytosolic M14 metallocarboxypeptidase (CCP) from Pseudomonas aeruginosa: a model for mammalian CCPs. The novel structure of a cytosolic M14 metallocarboxypeptidase (CCP) from Pseudomonas aeruginosa: a model for mammalian CCPs.,Otero A, Rodriguez de la Vega M, Tanco S, Lorenzo J, Aviles FX, Reverter D FASEB J. 2012 May 29. PMID:22645247[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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