1s00: Difference between revisions

Revision as of 08:07, 3 May 2008

PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG M233 REPLACED WITH CYS IN THE CHARGE-SEPARATED D+QAQB- STATE

OverviewOverview

In the photosynthetic reaction center (RC) from Rhodobacter sphaeroides, the reduction of a bound quinone molecule Q(B) is coupled with proton uptake. When Asp-L213 is replaced by Asn, proton transfer is inhibited. Proton transfer was restored by two second-site revertant mutations, Arg-M233-->Cys and Arg-H177-->His. Kinetic effects of Cd(2+) on proton transfer showed that the entry point in revertant RCs to be the same as in the native RC. The structures of the parental and two revertant RCs were determined at resolutions of 2.10, 1.80, and 2.75 A. From the structures, we were able to delineate alternate proton transfer pathways in the revertants. The main changes occur near Glu-H173, which allow it to substitute for the missing Asp-L213. The electrostatic changes near Glu-H173 cause it to be a good proton donor and acceptor, and the structural changes create a cavity which accommodates water molecules that connect Glu-H173 to other proton transfer components.

About this StructureAbout this Structure

1S00 is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

ReferenceReference

X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways., Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G, Structure. 2004 Apr;12(4):703-15. PMID:15062092 Page seeded by OCA on Sat May 3 08:07:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1s00.gif|left|200px]]
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-|GENE= PUFL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), PUFM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), PUHA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides])
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s00 OCA], [http://www.ebi.ac.uk/pdbsum/1s00 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s00 RCSB]</span>
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 '''PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG M233 REPLACED WITH CYS IN THE CHARGE-SEPARATED D+QAQB- STATE'''
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 [[Category: Paddock, M L.]]
 [[Category: Xu, Q.]]
-[[Category: bacterial photosynthesis]]
+[[Category: Bacterial photosynthesis]]
-[[Category: integral membrane protein]]
+[[Category: Integral membrane protein]]
-[[Category: proton transfer pathway]]
+[[Category: Proton transfer pathway]]
-[[Category: revertant]]
+[[Category: Revertant]]
-[[Category: rhodobacter sphaeroide]]
+[[Category: Rhodobacter sphaeroide]]
-[[Category: x-ray crystallography]]
+[[Category: X-ray crystallography]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:07:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:35:24 2008''