1rwj: Difference between revisions

Revision as of 07:59, 3 May 2008

c7-type three-heme cytochrome domain

OverviewOverview

The structure of a novel c(7)-type cytochrome domain that has two bishistidine coordinated hemes and one heme with histidine, methionine coordination (where the sixth ligand is a methionine residue) was determined at 1.7 A resolution. This domain is a representative of domains that form three polymers encoded by the Geobacter sulfurreducens genome. Two of these polymers consist of four and one protein of nine c(7)-type domains with a total of 12 and 27 hemes, respectively. Four individual domains (termed A, B, C, and D) from one such multiheme cytochrome c (ORF03300) were cloned and expressed in Escherichia coli. The domain C produced diffraction quality crystals from 2.4 M sodium malonate (pH 7). The structure was solved by MAD method and refined to an R-factor of 19.5% and R-free of 21.8%. Unlike the two c(7) molecules with known structures, one from G. sulfurreducens (PpcA) and one from Desulfuromonas acetoxidans where all three hemes are bishistidine coordinated, this domain contains a heme which is coordinated by a methionine and a histidine residue. As a result, the corresponding heme could have a higher potential than the other two hemes. The apparent midpoint reduction potential, E(app), of domain C is -105 mV, 50 mV higher than that of PpcA.

About this StructureAbout this Structure

1RWJ is a Single protein structure of sequence from Geobacter sulfurreducens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a novel c7-type three-heme cytochrome domain from a multidomain cytochrome c polymer., Pokkuluri PR, Londer YY, Duke NE, Erickson J, Pessanha M, Salgueiro CA, Schiffer M, Protein Sci. 2004 Jun;13(6):1684-92. Epub 2004 May 7. PMID:15133162 Page seeded by OCA on Sat May 3 07:59:29 2008

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OCA

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 [[Image:1rwj.gif|left|200px]]
- {{Structure
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-|GENE= ORF03300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35554 Geobacter sulfurreducens])
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-|RELATEDENTRY=[[1os6|1os6]], [[1hh5|1hh5]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwj OCA], [http://www.ebi.ac.uk/pdbsum/1rwj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rwj RCSB]</span>
-}}
 '''c7-type three-heme cytochrome domain'''
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 [[Category: Salgueiro, C A.]]
 [[Category: Schiffer, M.]]
-[[Category: cytochrome c7]]
+[[Category: Cytochrome c7]]
-[[Category: geobacter metallireducen]]
+[[Category: Geobacter metallireducen]]
-[[Category: geobacter sulfurreducen]]
+[[Category: Geobacter sulfurreducen]]
-[[Category: heme coordination in c-type cytochrome]]
+[[Category: Heme coordination in c-type cytochrome]]
-[[Category: multiheme cytochrome c]]
+[[Category: Multiheme cytochrome c]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:59:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:33:54 2008''