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==NMR Structure of the CRIB-PDZ module of Par-6==
==NMR Structure of the CRIB-PDZ module of Par-6==
<StructureSection load='1ry4' size='340' side='right'caption='[[1ry4]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1ry4' size='340' side='right'caption='[[1ry4]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1ry4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RY4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1ry4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RY4 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Par-6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ry4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ry4 OCA], [https://pdbe.org/1ry4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ry4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ry4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ry4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ry4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ry4 OCA], [https://pdbe.org/1ry4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ry4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ry4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ry4 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O97111_DROME O97111_DROME]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ry4 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ry4 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Regulation of protein interaction domains is required for cellular signaling dynamics. Here, we show that the PDZ protein interaction domain from the cell polarity protein Par-6 is regulated by the Rho GTPase Cdc42. Cdc42 binds to a CRIB domain adjacent to the PDZ domain, increasing the affinity of the Par-6 PDZ for its carboxy-terminal ligand by approximately 13-fold. Par-6 PDZ regulation is required for function as mutational disruption of Cdc42-Par-6 PDZ coupling leads to inactivation of Par-6 in polarized MDCK epithelial cells. Structural analysis reveals that the free PDZ domain has several deviations from the canonical PDZ conformation that account for its low ligand affinity. Regulation results from a Cdc42-induced conformational transition in the CRIB-PDZ module that causes the PDZ to assume a canonical, high-affinity PDZ conformation. The coupled CRIB and PDZ architecture of Par-6 reveals how simple binding domains can be combined to yield complex regulation.
Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition.,Peterson FC, Penkert RR, Volkman BF, Prehoda KE Mol Cell. 2004 Mar 12;13(5):665-76. PMID:15023337<ref>PMID:15023337</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ry4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Drome]]
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Penkert, R R]]
[[Category: Penkert RR]]
[[Category: Peterson, F C]]
[[Category: Peterson FC]]
[[Category: Prehoda, K E]]
[[Category: Prehoda KE]]
[[Category: Volkman, B F]]
[[Category: Volkman BF]]
[[Category: Cdc-42]]
[[Category: Cell adhesion]]
[[Category: Cell polarization]]
[[Category: Crib]]
[[Category: Pdz]]
[[Category: Polarity adaptor complex]]

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