1rwj: Difference between revisions

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<StructureSection load='1rwj' size='340' side='right'caption='[[1rwj]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='1rwj' size='340' side='right'caption='[[1rwj]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1rwj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_51573 Atcc 51573]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RWJ FirstGlance]. <br>
<table><tr><td colspan='2'>[[1rwj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RWJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1os6|1os6]], [[1hh5|1hh5]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF03300 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35554 ATCC 51573])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwj OCA], [https://pdbe.org/1rwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rwj RCSB], [https://www.ebi.ac.uk/pdbsum/1rwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rwj ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwj OCA], [https://pdbe.org/1rwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rwj RCSB], [https://www.ebi.ac.uk/pdbsum/1rwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rwj ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q74BP5_GEOSL Q74BP5_GEOSL]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rwj ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rwj ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of a novel c(7)-type cytochrome domain that has two bishistidine coordinated hemes and one heme with histidine, methionine coordination (where the sixth ligand is a methionine residue) was determined at 1.7 A resolution. This domain is a representative of domains that form three polymers encoded by the Geobacter sulfurreducens genome. Two of these polymers consist of four and one protein of nine c(7)-type domains with a total of 12 and 27 hemes, respectively. Four individual domains (termed A, B, C, and D) from one such multiheme cytochrome c (ORF03300) were cloned and expressed in Escherichia coli. The domain C produced diffraction quality crystals from 2.4 M sodium malonate (pH 7). The structure was solved by MAD method and refined to an R-factor of 19.5% and R-free of 21.8%. Unlike the two c(7) molecules with known structures, one from G. sulfurreducens (PpcA) and one from Desulfuromonas acetoxidans where all three hemes are bishistidine coordinated, this domain contains a heme which is coordinated by a methionine and a histidine residue. As a result, the corresponding heme could have a higher potential than the other two hemes. The apparent midpoint reduction potential, E(app), of domain C is -105 mV, 50 mV higher than that of PpcA.
Structure of a novel c7-type three-heme cytochrome domain from a multidomain cytochrome c polymer.,Pokkuluri PR, Londer YY, Duke NE, Erickson J, Pessanha M, Salgueiro CA, Schiffer M Protein Sci. 2004 Jun;13(6):1684-92. Epub 2004 May 7. PMID:15133162<ref>PMID:15133162</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1rwj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 51573]]
[[Category: Geobacter sulfurreducens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Duke, N E.C]]
[[Category: Duke NEC]]
[[Category: Erickson, J]]
[[Category: Erickson J]]
[[Category: Londer, Y Y]]
[[Category: Londer YY]]
[[Category: Pessanha, M]]
[[Category: Pessanha M]]
[[Category: Pokkuluri, P R]]
[[Category: Pokkuluri PR]]
[[Category: Salgueiro, C A]]
[[Category: Salgueiro CA]]
[[Category: Schiffer, M]]
[[Category: Schiffer M]]
[[Category: Cytochrome c7]]
[[Category: Electron transport]]
[[Category: Geobacter metallireducen]]
[[Category: Geobacter sulfurreducen]]
[[Category: Heme coordination in c-type cytochrome]]
[[Category: Multiheme cytochrome c]]

Revision as of 11:27, 1 May 2024

c7-type three-heme cytochrome domainc7-type three-heme cytochrome domain

Structural highlights

1rwj is a 1 chain structure with sequence from Geobacter sulfurreducens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q74BP5_GEOSL

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1rwj, resolution 1.70Å

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OCA
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