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| ==Structure of h. salinarum RosR (vng0258) grown from NaCl== | | ==Structure of h. salinarum RosR (vng0258) grown from NaCl== |
| <StructureSection load='6f5c' size='340' side='right' caption='[[6f5c]], [[Resolution|resolution]] 1.55Å' scene=''> | | <StructureSection load='6f5c' size='340' side='right'caption='[[6f5c]], [[Resolution|resolution]] 1.55Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6f5c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Halsa Halsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F5C FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6f5c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum_NRC-1 Halobacterium salinarum NRC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F5C FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ez1|6ez1]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VNG_0258H ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64091 HALSA])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f5c OCA], [https://pdbe.org/6f5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f5c RCSB], [https://www.ebi.ac.uk/pdbsum/6f5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f5c ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f5c OCA], [http://pdbe.org/6f5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f5c RCSB], [http://www.ebi.ac.uk/pdbsum/6f5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f5c ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q9HSF4_HALSA Q9HSF4_HALSA] |
| Protein-DNA interactions are highly dependent on salt concentration. To gain insight into how such interactions are maintained in the highly saline cytoplasm of halophilic archaea, we determined the 3-D structure of VNG0258H/RosR, the first haloarchaeal DNA-binding protein from the extreme halophilic archaeon Halobactrium salinarum. It is a dimeric winged-helix-turn-helix (wHTH) protein with unique features due to adaptation to the halophilic environment. As ions are major players in DNA binding processes, particularly in halophilic environments, we investigated the solution structure of the ionic envelope and located anions in the first shell around the protein in the crystal using anomalous scattering. Anions that were found to be tightly bound to residues in the positively charged DNA-binding site would probably be released upon DNA binding and will thus make significant contribution to the driving force of the binding process. Unexpectedly, ions were also found in a buried internal cavity connected to the external medium by a tunnel. Our structure lays a solid groundwork for future structural, computational and biochemical studies on complexes of the protein with cognate DNA sequences, with implications to protein-DNA interactions in hyper-saline environments.
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| The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.,Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B J Struct Biol. 2018 Aug 12. pii: S1047-8477(18)30226-0. doi:, 10.1016/j.jsb.2018.08.008. PMID:30110657<ref>PMID:30110657</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6f5c" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Halsa]] | | [[Category: Halobacterium salinarum NRC-1]] |
| [[Category: Kutnowski, N]] | | [[Category: Large Structures]] |
| [[Category: Shaanan, B]] | | [[Category: Kutnowski N]] |
| [[Category: Shmuely, H]] | | [[Category: Shaanan B]] |
| [[Category: Dna binding protein]] | | [[Category: Shmuely H]] |
| [[Category: Halophile]]
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| [[Category: High salt medium]]
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| [[Category: Rosr]]
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| [[Category: Whth dna binding protein]]
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