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| == Function == | | == Function == |
| [https://www.uniprot.org/uniprot/Q9Q1V2_9VIRU Q9Q1V2_9VIRU] | | [https://www.uniprot.org/uniprot/Q9Q1V2_9VIRU Q9Q1V2_9VIRU] |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Double-stranded (ds) RNA virus particles are organized around a central icosahedral core capsid made of 120 identical subunits. This core capsid is unable to invade cells from outside, and animal dsRNA viruses have acquired surrounding capsid layers that are used to deliver a transcriptionally active core particle across the membrane during cell entry. In contrast, dsRNA viruses infecting primitive eukaryotes have only a simple core capsid, and as a consequence are transmitted only vertically. Here, we report the 3.4 A X-ray structure of a picobirnavirus--an animal dsRNA virus associated with diarrhoea and gastroenteritis in humans. The structure shows a simple core capsid with a distinctive icosahedral arrangement, displaying 60 two-fold symmetric dimers of a coat protein (CP) with a new 3D-fold. We show that, as many non-enveloped animal viruses, CP undergoes an autoproteolytic cleavage, releasing a post-translationally modified peptide that remains associated with nucleic acid within the capsid. Our data also show that picobirnavirus particles are capable of disrupting biological membranes in vitro, indicating that its simple 120-subunits capsid has evolved animal cell invasion properties.
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| The picobirnavirus crystal structure provides functional insights into virion assembly and cell entry.,Duquerroy S, Da Costa B, Henry C, Vigouroux A, Libersou S, Lepault J, Navaza J, Delmas B, Rey FA EMBO J. 2009 Jun 3;28(11):1655-65. Epub 2009 Apr 30. PMID:19407816<ref>PMID:19407816</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2vf1" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] | | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |