1rod: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1rod.gif|left|200px]] | [[Image:1rod.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1rod", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1rod| PDB=1rod | SCENE= }} | |||
| | |||
}} | |||
'''CHIMERIC PROTEIN OF INTERLEUKIN 8 AND HUMAN MELANOMA GROWTH STIMULATING ACTIVITY PROTEIN, NMR''' | '''CHIMERIC PROTEIN OF INTERLEUKIN 8 AND HUMAN MELANOMA GROWTH STIMULATING ACTIVITY PROTEIN, NMR''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ROD OCA]. | |||
==Reference== | ==Reference== | ||
Structure and activity of a chimeric interleukin-8-melanoma-growth-stimulatory-activity protein., Sticht H, Auer M, Schmitt B, Besemer J, Horcher M, Kirsch T, Lindley IJ, Rosch P, Eur J Biochem. 1996 Jan 15;235(1-2):26-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8631339 8631339] | Structure and activity of a chimeric interleukin-8-melanoma-growth-stimulatory-activity protein., Sticht H, Auer M, Schmitt B, Besemer J, Horcher M, Kirsch T, Lindley IJ, Rosch P, Eur J Biochem. 1996 Jan 15;235(1-2):26-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8631339 8631339] | ||
[[Category: Auer, M.]] | [[Category: Auer, M.]] | ||
[[Category: Besemer, J.]] | [[Category: Besemer, J.]] | ||
| Line 33: | Line 28: | ||
[[Category: Schmitt, B.]] | [[Category: Schmitt, B.]] | ||
[[Category: Sticht, H.]] | [[Category: Sticht, H.]] | ||
[[Category: | [[Category: Chemokine]] | ||
[[Category: | [[Category: Chemotaxis]] | ||
[[Category: | [[Category: Cytokine]] | ||
[[Category: | [[Category: Inflammatory response]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:43:37 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 07:43, 3 May 2008
CHIMERIC PROTEIN OF INTERLEUKIN 8 AND HUMAN MELANOMA GROWTH STIMULATING ACTIVITY PROTEIN, NMR
OverviewOverview
A 72-amino-acid chimeric protein, Chi1, was constructed from the N-terminal part of interleukin 8, IL-8-(1-53), and the C-terminal part of melanoma growth stimulatory activity, MGSA-(54-72). Chi1 protein showed receptor-binding specificity and biological activity similar, but not identical to IL-8 and decidedly different from MGSA. The structure of Chi1 was determined in solution by two-dimensional NMR and molecular-dynamics calculations. The structure resembled the structures of MGSA and IL-8 closely, containing a triple-stranded beta-sheet in the IL-8 part and an amphipathic alpha-helix in the MGSA part. Chi1 formed dimers at millimolar concentrations via the first strand from the N-terminus, analogous to IL-8 and MGSA. In contrast to the latter molecules, however, the alpha-helix of Chi1 did not pack against the beta-sheet part, but was an independent structural element. This structural difference could be explained mainly by the modulation of hydrophobic interactions between the helix and the rest of the protein in Chi1 as compared to IL-8 and MGSA. It is concluded that tight helix packing is not required for receptor binding and biological activity of Chi1.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Structure and activity of a chimeric interleukin-8-melanoma-growth-stimulatory-activity protein., Sticht H, Auer M, Schmitt B, Besemer J, Horcher M, Kirsch T, Lindley IJ, Rosch P, Eur J Biochem. 1996 Jan 15;235(1-2):26-35. PMID:8631339 Page seeded by OCA on Sat May 3 07:43:37 2008