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==Solution structure of human mitochondria fission protein Fis1==
==Solution structure of human mitochondria fission protein Fis1==
<StructureSection load='1pc2' size='340' side='right'caption='[[1pc2]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1pc2' size='340' side='right'caption='[[1pc2]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1pc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PC2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1pc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PC2 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Fis1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pc2 OCA], [https://pdbe.org/1pc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pc2 RCSB], [https://www.ebi.ac.uk/pdbsum/1pc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pc2 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pc2 OCA], [https://pdbe.org/1pc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pc2 RCSB], [https://www.ebi.ac.uk/pdbsum/1pc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pc2 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/FIS1_HUMAN FIS1_HUMAN]] Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.<ref>PMID:12783892</ref> <ref>PMID:12861026</ref> <ref>PMID:14996942</ref> <ref>PMID:16118244</ref> <ref>PMID:16107562</ref>
[https://www.uniprot.org/uniprot/FIS1_HUMAN FIS1_HUMAN] Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.<ref>PMID:12783892</ref> <ref>PMID:12861026</ref> <ref>PMID:14996942</ref> <ref>PMID:16118244</ref> <ref>PMID:16107562</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pc2 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pc2 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fis1 in yeast localizes to the outer mitochondrial membrane and facilitates mitochondrial fission by forming protein complexes with Dnm1 and Mdv1. Fis1 orthologs exist in higher eukaryotes, suggesting that they are functionally conserved. In the present study, we cloned the human Fis1 ortholog that was predicted in a database, and determined the protein structure using NMR spectroscopy. Following a flexible N-terminal tail, six alpha-helices connected with short loops construct a single core domain. The C-terminal tail containing a transmembrane segment appears to be disordered. In the core domain, each of two sequentially adjacent helices forms a hairpin-like conformation, resulting in a six helix assembly forming a slightly twisted slab similar to that of a tandem array of tetratrico-peptide repeat (TPR) motif folds. Within this TPR-like core domain, no significant sequence similarity to the typical TPR motif is found. The structural analogy to the TPR-containing proteins suggests that Fis1 binds to other proteins at its concave hydrophobic surface. A simple composition of Fis1 comprised of a binding domain and a transmembrane segment indicates that the protein may function as a molecular adaptor on the mitochondrial outer membrane. In HeLa cells, however, increased levels in mitochondria-associated Fis1 did not result in mitochondrial translocation of Drp1, a potential binding partner of Fis1 implicated in the regulation of mitochondrial fission, suggesting that the interaction between Drp1 and Fis1 is regulated.
The solution structure of human mitochondria fission protein Fis1 reveals a novel TPR-like helix bundle.,Suzuki M, Jeong SY, Karbowski M, Youle RJ, Tjandra N J Mol Biol. 2003 Nov 28;334(3):445-58. PMID:14623186<ref>PMID:14623186</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1pc2" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Suzuki, M]]
[[Category: Suzuki M]]
[[Category: Tjandra, N]]
[[Category: Tjandra N]]
[[Category: Youle, R J]]
[[Category: Youle RJ]]
[[Category: Fission]]
[[Category: Mitochondria]]
[[Category: Unknown function]]

Revision as of 08:54, 17 April 2024

Solution structure of human mitochondria fission protein Fis1Solution structure of human mitochondria fission protein Fis1

Structural highlights

1pc2 is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FIS1_HUMAN Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.[1] [2] [3] [4] [5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ James DI, Parone PA, Mattenberger Y, Martinou JC. hFis1, a novel component of the mammalian mitochondrial fission machinery. J Biol Chem. 2003 Sep 19;278(38):36373-9. Epub 2003 Jun 3. PMID:12783892 doi:http://dx.doi.org/10.1074/jbc.M303758200
  2. ↑ Yoon Y, Krueger EW, Oswald BJ, McNiven MA. The mitochondrial protein hFis1 regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1. Mol Cell Biol. 2003 Aug;23(15):5409-20. PMID:12861026
  3. ↑ Stojanovski D, Koutsopoulos OS, Okamoto K, Ryan MT. Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphology. J Cell Sci. 2004 Mar 1;117(Pt 7):1201-10. PMID:14996942 doi:http://dx.doi.org/10.1242/jcs.01058
  4. ↑ Yu T, Fox RJ, Burwell LS, Yoon Y. Regulation of mitochondrial fission and apoptosis by the mitochondrial outer membrane protein hFis1. J Cell Sci. 2005 Sep 15;118(Pt 18):4141-51. Epub 2005 Aug 23. PMID:16118244 doi:http://dx.doi.org/jcs.02537
  5. ↑ Koch A, Yoon Y, Bonekamp NA, McNiven MA, Schrader M. A role for Fis1 in both mitochondrial and peroxisomal fission in mammalian cells. Mol Biol Cell. 2005 Nov;16(11):5077-86. Epub 2005 Aug 17. PMID:16107562 doi:http://dx.doi.org/E05-02-0159
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