8dx0: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8dx0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus Enterococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DX0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[8dx0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus Enterococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DX0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dx0 OCA], [https://pdbe.org/8dx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dx0 RCSB], [https://www.ebi.ac.uk/pdbsum/8dx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dx0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dx0 OCA], [https://pdbe.org/8dx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dx0 RCSB], [https://www.ebi.ac.uk/pdbsum/8dx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dx0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/Q9KJT3_ENTGA Q9KJT3_ENTGA]  
[https://www.uniprot.org/uniprot/Q9KJT3_ENTGA Q9KJT3_ENTGA]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The VanRS two-component system regulates the resistance phenotype of vancomycin-resistant enterococci. VanS is a sensor histidine kinase that responds to the presence of vancomycin by autophosphorylating and subsequently transferring the phosphoryl group to the response regulator, VanR. The phosphotransfer activates VanR as a transcription factor, which initiates the expression of resistance genes. Structural information about VanS proteins has remained elusive, hindering the molecular-level understanding of their function. Here, we present X-ray crystal structures for the catalytic and ATP-binding (CA) domains of two VanS proteins, derived from vancomycin-resistant enterococci types A and C. Both proteins adopt the canonical Bergerat fold that has been observed for CA domains of other prokaryotic histidine kinases. We attempted to determine structures for the nucleotide-bound forms of both proteins; however, despite repeated efforts, these forms could not be crystallized, prompting us to measure the proteins' binding affinities for ATP. Unexpectedly, both CA domains displayed low affinities for the nucleotide, with K(D) values in the low millimolar range. Since these K(D) values are comparable to intracellular ATP concentrations, this weak substrate binding could reflect a way of regulating expression of the resistance phenotype.
Structure of VanS from vancomycin-resistant enterococci: A sensor kinase with weak ATP binding.,Grasty KC, Guzik C, D'Lauro EJ, Padrick SB, Beld J, Loll PJ J Biol Chem. 2023 Feb 9;299(3):103001. doi: 10.1016/j.jbc.2023.103001. PMID:36764524<ref>PMID:36764524</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8dx0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 10:25, 3 April 2024

VanSC CA domainVanSC CA domain

Structural highlights

8dx0 is a 2 chain structure with sequence from Enterococcus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.45Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9KJT3_ENTGA

8dx0, resolution 1.45Å

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