1rh9: Difference between revisions

Revision as of 07:29, 3 May 2008

Family GH5 endo-beta-mannanase from Lycopersicon esculentum (tomato)

OverviewOverview

The three-dimensional crystal structure of tomato (Lycopersicon esculentum) beta-mannanase 4a (LeMAN4a) has been determined to 1.5 A resolution. The enzyme adopts the (beta/alpha)(8) fold common to the members of glycohydrolase family GH5. The structure is comparable with those of the homologous Trichoderma reesei and Thermomonospora fusca beta-mannanases: There is a conserved three-stranded beta-sheet located near the N terminus that stacks against the central beta-barrel at the end opposite the active site. Three noncanonical beta-helices surround the active site. Similar helices are found in T. reesei but not T. fusca beta-mannanase. By analogy with other beta-mannanases, the catalytic acid/base residue is E204 and the nucleophile residue is E318. The active site cleft of L. esculentum beta-mannanase most closely resembles that of the T. reesei isozyme. A model of substrate binding in LeMAN4a is proposed in which the mannosyl residue occupying the -1 subsite of the enzyme adopts the (1)S(5) skew-boat conformation.

About this StructureAbout this Structure

1RH9 is a Single protein structure of sequence from Solanum lycopersicum. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of (1,4)-beta-D-mannan mannanohydrolase from tomato fruit., Bourgault R, Oakley AJ, Bewley JD, Wilce MC, Protein Sci. 2005 May;14(5):1233-41. PMID:15840830 Page seeded by OCA on Sat May 3 07:29:50 2008

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OCA

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 [[Image:1rh9.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1rh9 |SIZE=350|CAPTION= <scene name='initialview01'>1rh9</scene>, resolution 1.50&Aring;
+The line below this paragraph, containing "STRUCTURE_1rh9", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= LeMAN4a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4081 Solanum lycopersicum])
+-->
-|DOMAIN=
+{{STRUCTURE_1rh9|  PDB=1rh9  |  SCENE=  }}
-|RELATEDENTRY=[[1bqc|1BQC]], [[1qnp|1QNP]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh9 OCA], [http://www.ebi.ac.uk/pdbsum/1rh9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rh9 RCSB]</span>
-}}
 '''Family GH5 endo-beta-mannanase from Lycopersicon esculentum (tomato)'''
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 [[Category: Oakley, A J.]]
 [[Category: Wilce, M C.J.]]
-[[Category: endo-beta-mannase]]
+[[Category: Endo-beta-mannase]]
-[[Category: glycoside hydrolase family 5]]
+[[Category: Glycoside hydrolase family 5]]
-[[Category: mannan]]
+[[Category: Mannan]]
-[[Category: retaining]]
+[[Category: Retaining]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:29:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:28:08 2008''