1rf8: Difference between revisions

Revision as of 07:25, 3 May 2008

Solution structure of the yeast translation initiation factor eIF4E in complex with m7GDP and eIF4GI residues 393 to 490

OverviewOverview

The eukaryotic initiation factor 4G (eIF4G) is the core of a multicomponent switch controlling gene expression at the level of translation initiation. It interacts with the small ribosomal subunit interacting protein, eIF3, and the eIF4E/cap-mRNA complex in order to load the ribosome onto mRNA during cap-dependent translation. We describe the solution structure of the complex between yeast eIF4E/cap and eIF4G (393-490). Binding triggers a coupled folding transition of eIF4G (393-490) and the eIF4E N terminus resulting in a molecular bracelet whereby eIF4G (393-490) forms a right-handed helical ring that wraps around the N terminus of eIF4E. Cofolding allosterically enhances association of eIF4E with the cap and is required for maintenance of optimal growth and polysome distributions in vivo. Our data explain how mRNA, eIF4E, and eIF4G exists as a stable mRNP that may facilitate multiple rounds of ribosomal loading during translation initiation, a key determinant in the overall rate of protein synthesis.

About this StructureAbout this Structure

1RF8 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Ribosome loading onto the mRNA cap is driven by conformational coupling between eIF4G and eIF4E., Gross JD, Moerke NJ, von der Haar T, Lugovskoy AA, Sachs AB, McCarthy JE, Wagner G, Cell. 2003 Dec 12;115(6):739-50. PMID:14675538 Page seeded by OCA on Sat May 3 07:25:31 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1rf8.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1rf8", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|GENE= TIF45, CDC33, YOL139C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), TIF4631, YGR162W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
+{{STRUCTURE_1rf8|  PDB=1rf8  |  SCENE=  }}
-|RELATEDENTRY=[[1ap8|1AP8]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rf8 OCA], [http://www.ebi.ac.uk/pdbsum/1rf8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rf8 RCSB]</span>
-}}
 '''Solution structure of the yeast translation initiation factor eIF4E in complex with m7GDP and eIF4GI residues 393 to 490'''
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 [[Category: Sachs, A B.]]
 [[Category: Wagner, G.]]
-[[Category: initiation factor]]
+[[Category: Initiation factor]]
-[[Category: protein biosynthesis]]
+[[Category: Protein biosynthesis]]
-[[Category: translation regulation]]
+[[Category: Translation regulation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:25:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:27:23 2008''