7d7d: Difference between revisions

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====
==CryoEM structure of gp45-dependent transcription activation complex==
<StructureSection load='7d7d' size='340' side='right'caption='[[7d7d]]' scene=''>
<StructureSection load='7d7d' size='340' side='right'caption='[[7d7d]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
<table><tr><td colspan='2'>[[7d7d]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Escherichia_coli_1-392-07_S4_C3 Escherichia coli 1-392-07_S4_C3] and [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D7D FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d7d OCA], [https://pdbe.org/7d7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d7d RCSB], [https://www.ebi.ac.uk/pdbsum/7d7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d7d ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d7d OCA], [https://pdbe.org/7d7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d7d RCSB], [https://www.ebi.ac.uk/pdbsum/7d7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d7d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CLAMP_BPT4 CLAMP_BPT4] Sliding clamp that encircles the genomic DNA and links the DNA polymerase to the template to control the processivity of DNA synthesis. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication (PubMed:10535942). Interaction with the sliding-clamp-loader opens the sliding clamp so that it can be loaded around the DNA template (PubMed:22194570). During transcription, encircles the DNA and tethers host RNA polymerase (RNAP) to it (PubMed:33602900).[HAMAP-Rule:MF_04161]<ref>PMID:10535942</ref> <ref>PMID:22194570</ref> <ref>PMID:33602900</ref>
==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli 1-392-07_S4_C3]]
[[Category: Escherichia virus T4]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Z-disk]]
[[Category: Feng Y]]
[[Category: Jin S]]
[[Category: Shi J]]
[[Category: Wen A]]

Latest revision as of 13:53, 27 March 2024

CryoEM structure of gp45-dependent transcription activation complexCryoEM structure of gp45-dependent transcription activation complex

Structural highlights

7d7d is a 12 chain structure with sequence from Escherichia coli, Escherichia coli 1-392-07_S4_C3 and Escherichia virus T4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 4.5Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CLAMP_BPT4 Sliding clamp that encircles the genomic DNA and links the DNA polymerase to the template to control the processivity of DNA synthesis. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication (PubMed:10535942). Interaction with the sliding-clamp-loader opens the sliding clamp so that it can be loaded around the DNA template (PubMed:22194570). During transcription, encircles the DNA and tethers host RNA polymerase (RNAP) to it (PubMed:33602900).[HAMAP-Rule:MF_04161][1] [2] [3]

See Also

References

  1. ↑ Latham GJ, Dong F, Pietroni P, Dozono JM, Bacheller DJ, von Hippel PH. Opening of a monomer-monomer interface of the trimeric bacteriophage T4-coded GP45 sliding clamp is required for clamp loading onto DNA. Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12448-53. PMID:10535942 doi:10.1073/pnas.96.22.12448
  2. ↑ Kelch BA, Makino DL, O'Donnell M, Kuriyan J. How a DNA polymerase clamp loader opens a sliding clamp. Science. 2011 Dec 23;334(6063):1675-80. PMID:22194570 doi:10.1126/science.1211884
  3. ↑ Shi J, Wen A, Jin S, Gao B, Huang Y, Feng Y. Transcription activation by a sliding clamp. Nat Commun. 2021 Feb 18;12(1):1131. PMID:33602900 doi:10.1038/s41467-021-21392-0

7d7d, resolution 4.50Å

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