7bvf: Difference between revisions

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<StructureSection load='7bvf' size='340' side='right'caption='[[7bvf]], [[Resolution|resolution]] 2.97&Aring;' scene=''>
<StructureSection load='7bvf' size='340' side='right'caption='[[7bvf]], [[Resolution|resolution]] 2.97&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7bvf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu] and [http://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_mc2_155 Mycolicibacterium smegmatis mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BVF FirstGlance]. <br>
<table><tr><td colspan='2'>[[7bvf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv] and [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BVF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=95E:Ethambutol'>95E</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=DSL:MONO-TRANS,+OCTA-CIS+DECAPRENYL-PHOSPHATE'>DSL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.97&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">embB, Rv3795, MTCY13D12.29 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), embA, Rv3794, MTCY13D12.28 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=95E:Ethambutol'>95E</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=DSL:MONO-TRANS,+OCTA-CIS+DECAPRENYL-PHOSPHATE'>DSL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvf OCA], [http://pdbe.org/7bvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bvf RCSB], [http://www.ebi.ac.uk/pdbsum/7bvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvf ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvf OCA], [https://pdbe.org/7bvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bvf RCSB], [https://www.ebi.ac.uk/pdbsum/7bvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvf ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/EMBB_MYCTU EMBB_MYCTU]] Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan. [[http://www.uniprot.org/uniprot/ACPM_MYCS2 ACPM_MYCS2]] Acyl carrier protein involved in meromycolate extension. [[http://www.uniprot.org/uniprot/EMBA_MYCTU EMBA_MYCTU]] Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
[https://www.uniprot.org/uniprot/EMBB_MYCTU EMBB_MYCTU] Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The arabinosyltransferases EmbA, EmbB, and EmbC are involved in Mycobacterium tuberculosis cell wall synthesis and are recognized as the targets for the anti-tuberculosis drug ethambutol. We have determined cryo-electron microscopy and x-ray crystal structures of mycobacterial EmbA-EmbB and EmbC-EmbC complexes, in the presence of their glycosyl donor and acceptor substrates and with ethambutol. These structures show how the donor and acceptor substrates bind in the active site and how ethambutol inhibits by binding to the same site as both substrates in EmbB and EmbC. The majority of drug-resistant mutations are located nearby to the ethambutol-binding site. Collectively, our work provides a structural basis for understanding the biochemical function and inhibition of arabinosyltransferases and development of new anti-tuberculosis agents.
 
Structures of cell wall arabinosyltransferases with the anti-tuberculosis drug ethambutol.,Zhang L, Zhao Y, Gao Y, Wu L, Gao R, Zhang Q, Wang Y, Wu C, Wu F, Gurcha SS, Veerapen N, Batt SM, Zhao W, Qin L, Yang X, Wang M, Zhu Y, Zhang B, Bi L, Zhang X, Yang H, Guddat LW, Xu W, Wang Q, Li J, Besra GS, Rao Z Science. 2020 Apr 23. pii: science.aba9102. doi: 10.1126/science.aba9102. PMID:32327601<ref>PMID:32327601</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7bvf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mycolicibacterium smegmatis mc2 155]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Myctu]]
[[Category: Mycolicibacterium smegmatis MC2 155]]
[[Category: Besra, G S]]
[[Category: Besra GS]]
[[Category: Gao, Y]]
[[Category: Gao Y]]
[[Category: Li, J]]
[[Category: Li J]]
[[Category: Rao, Z]]
[[Category: Rao Z]]
[[Category: Wang, Q]]
[[Category: Wang Q]]
[[Category: Zhang, L]]
[[Category: Zhang L]]
[[Category: Zhao, Y]]
[[Category: Zhao Y]]
[[Category: Acyl carrier protein]]
[[Category: Arabinogalactan]]
[[Category: Arabinosyltransferase]]
[[Category: Cell wall synthesis]]
[[Category: Drug resistance]]
[[Category: Drug target]]
[[Category: Emba]]
[[Category: Embb]]
[[Category: Embc]]
[[Category: Ethambutol]]
[[Category: Lipoarabinomannan]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Transferase]]

Latest revision as of 13:49, 27 March 2024

Cryo-EM structure of Mycobacterium tuberculosis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with ethambutolCryo-EM structure of Mycobacterium tuberculosis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with ethambutol

Structural highlights

7bvf is a 3 chain structure with sequence from Mycobacterium tuberculosis H37Rv and Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.97Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EMBB_MYCTU Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.

7bvf, resolution 2.97Å

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