6klw: Difference between revisions

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 <SX load='6klw' size='340' side='right' viewer='molstar' caption='[[6klw]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6klw]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KLW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KLW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6klw]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KLW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6klw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6klw OCA], [http://pdbe.org/6klw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6klw RCSB], [http://www.ebi.ac.uk/pdbsum/6klw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6klw ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6klw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6klw OCA], [https://pdbe.org/6klw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6klw RCSB], [https://www.ebi.ac.uk/pdbsum/6klw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6klw ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/Q46221_CLOPF Q46221_CLOPF]
-The iota toxin produced by Clostridium perfringens type E is a binary toxin comprising two independent polypeptides: Ia, an ADP-ribosyltransferase, and Ib, which is involved in cell binding and translocation of Ia across the cell membrane. Here we report cryo-EM structures of the translocation channel Ib-pore and its complex with Ia. The high-resolution Ib-pore structure demonstrates a similar structural framework to that of the catalytic varphi-clamp of the anthrax protective antigen pore. However, the Ia-bound Ib-pore structure shows a unique binding mode of Ia: one Ia binds to the Ib-pore, and the Ia amino-terminal domain forms multiple weak interactions with two additional Ib-pore constriction sites. Furthermore, Ib-binding induces tilting and partial unfolding of the Ia N-terminal alpha-helix, permitting its extension to the varphi-clamp gate. This new mechanism of N-terminal unfolding is crucial for protein translocation.
-Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex.,Yamada T, Yoshida T, Kawamoto A, Mitsuoka K, Iwasaki K, Tsuge H Nat Struct Mol Biol. 2020 Mar;27(3):288-296. doi: 10.1038/s41594-020-0388-6. Epub, 2020 Mar 2. PMID:32123390<ref>PMID:32123390</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 6klw" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </SX>
-[[Category: Bacillus perfringens veillon and zuber 1898]]
+[[Category: Clostridium perfringens]]
 [[Category: Large Structures]]
-[[Category: Iwasaki, K]]
+[[Category: Iwasaki K]]
-[[Category: Kawamoto, A]]
+[[Category: Kawamoto A]]
-[[Category: Mitsuoka, K]]
+[[Category: Mitsuoka K]]
-[[Category: Tsuge, H]]
+[[Category: Tsuge H]]
-[[Category: Yamada, T]]
+[[Category: Yamada T]]
-[[Category: Yoshida, T]]
+[[Category: Yoshida T]]
-[[Category: Adp-ribosylation]]
-[[Category: Bacterial binary toxin]]
-[[Category: Protein translocation channel]]
-[[Category: Toxin]]