6iew: Difference between revisions

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<StructureSection load='6iew' size='340' side='right'caption='[[6iew]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='6iew' size='340' side='right'caption='[[6iew]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6iew]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IEW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IEW FirstGlance]. <br>
<table><tr><td colspan='2'>[[6iew]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IEW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Panx, CG9754 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iew OCA], [http://pdbe.org/6iew PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iew RCSB], [http://www.ebi.ac.uk/pdbsum/6iew PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iew ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iew OCA], [https://pdbe.org/6iew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iew RCSB], [https://www.ebi.ac.uk/pdbsum/6iew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iew ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/NXF2_DROME NXF2_DROME]] May be involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:11780633</ref>
[https://www.uniprot.org/uniprot/NXF2_DROME NXF2_DROME] May be involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:11780633</ref> [https://www.uniprot.org/uniprot/PANX_DROME PANX_DROME] Acts via the piwi-interacting RNA (piRNA) pathway which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:26472911, PubMed:26494711). Required for transcriptional silencing of transposons targeted by piwi and confers its effects by interacting with nascent RNA transcripts (PubMed:26472911, PubMed:26494711). Likely to be recruited to nascent transcripts cotranscriptionally by piwi and to recruit additional factors involved in transcriptional silencing (PubMed:26472911). In the ovaries, forms a complex with nxf2, piwi and Nxt1 which acts as effectors of cotranscriptional transposon silencing (PubMed:31219034, PubMed:31368590, PubMed:31570835, PubMed:31384064). The interaction with nxf2 stabilizes the nuclear protein complex (PubMed:31384064).<ref>PMID:26472911</ref> <ref>PMID:26494711</ref> <ref>PMID:31219034</ref> <ref>PMID:31368590</ref> <ref>PMID:31384064</ref> <ref>PMID:31570835</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The repression of transposons by the Piwi-interacting RNA (piRNA) pathway is essential to protect animal germ cells. In Drosophila, Panoramix enforces transcriptional silencing by binding to the target-engaged Piwi-piRNA complex, although the precise mechanisms by which this occurs remain elusive. Here, we show that Panoramix functions together with a germline-specific paralogue of a nuclear export factor, dNxf2, and its cofactor dNxt1 (p15), to suppress transposon expression. The transposon RNA-binding protein dNxf2 is required for animal fertility and Panoramix-mediated silencing. Transient tethering of dNxf2 to nascent transcripts leads to their nuclear retention. The NTF2 domain of dNxf2 competes dNxf1 (TAP) off nucleoporins, a process required for proper RNA export. Thus, dNxf2 functions in a Panoramix-dNxf2-dependent TAP/p15 silencing (Pandas) complex that counteracts the canonical RNA exporting machinery and restricts transposons to the nuclear peripheries. Our findings may have broader implications for understanding how RNA metabolism modulates heterochromatin formation.
 
A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation.,Zhao K, Cheng S, Miao N, Xu P, Lu X, Zhang Y, Wang M, Ouyang X, Yuan X, Liu W, Lu X, Zhou P, Gu J, Zhang Y, Qiu D, Jin Z, Su C, Peng C, Wang JH, Dong MQ, Wan Y, Ma J, Cheng H, Huang Y, Yu Y Nat Cell Biol. 2019 Oct;21(10):1261-1272. doi: 10.1038/s41556-019-0396-0. Epub, 2019 Sep 30. PMID:31570835<ref>PMID:31570835</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6iew" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Drome]]
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cheng, S]]
[[Category: Cheng S]]
[[Category: Huang, Y]]
[[Category: Huang Y]]
[[Category: Pirna]]
[[Category: Protein binding]]

Latest revision as of 13:32, 27 March 2024

The crystal structure of the dNxf2 UBA domain in complex with PanoramixThe crystal structure of the dNxf2 UBA domain in complex with Panoramix

Structural highlights

6iew is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NXF2_DROME May be involved in the export of mRNA from the nucleus to the cytoplasm.[1] PANX_DROME Acts via the piwi-interacting RNA (piRNA) pathway which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:26472911, PubMed:26494711). Required for transcriptional silencing of transposons targeted by piwi and confers its effects by interacting with nascent RNA transcripts (PubMed:26472911, PubMed:26494711). Likely to be recruited to nascent transcripts cotranscriptionally by piwi and to recruit additional factors involved in transcriptional silencing (PubMed:26472911). In the ovaries, forms a complex with nxf2, piwi and Nxt1 which acts as effectors of cotranscriptional transposon silencing (PubMed:31219034, PubMed:31368590, PubMed:31570835, PubMed:31384064). The interaction with nxf2 stabilizes the nuclear protein complex (PubMed:31384064).[2] [3] [4] [5] [6] [7]

References

  1. ↑ Herold A, Klymenko T, Izaurralde E. NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila. RNA. 2001 Dec;7(12):1768-80. PMID:11780633
  2. ↑ Yu Y, Gu J, Jin Y, Luo Y, Preall JB, Ma J, Czech B, Hannon GJ. Panoramix enforces piRNA-dependent cotranscriptional silencing. Science. 2015 Oct 16;350(6258):339-42. PMID:26472911 doi:10.1126/science.aab0700
  3. ↑ Sienski G, Batki J, Senti KA, Dönertas D, Tirian L, Meixner K, Brennecke J. Silencio/CG9754 connects the Piwi-piRNA complex to the cellular heterochromatin machinery. Genes Dev. 2015 Nov 1;29(21):2258-71. PMID:26494711 doi:10.1101/gad.271908.115
  4. ↑ Fabry MH, Ciabrelli F, Munafò M, Eastwood EL, Kneuss E, Falciatori I, Falconio FA, Hannon GJ, Czech B. piRNA-guided co-transcriptional silencing coopts nuclear export factors. Elife. 2019 Jun 20;8:e47999. PMID:31219034 doi:10.7554/eLife.47999
  5. ↑ Murano K, Iwasaki YW, Ishizu H, Mashiko A, Shibuya A, Kondo S, Adachi S, Suzuki S, Saito K, Natsume T, Siomi MC, Siomi H. Nuclear RNA export factor variant initiates piRNA-guided co-transcriptional silencing. EMBO J. 2019 Sep 2;38(17):e102870. PMID:31368590 doi:10.15252/embj.2019102870
  6. ↑ Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J. The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nat Struct Mol Biol. 2019 Aug;26(8):720-731. doi: 10.1038/s41594-019-0270-6. Epub, 2019 Aug 5. PMID:31384064 doi:http://dx.doi.org/10.1038/s41594-019-0270-6
  7. ↑ Zhao K, Cheng S, Miao N, Xu P, Lu X, Zhang Y, Wang M, Ouyang X, Yuan X, Liu W, Lu X, Zhou P, Gu J, Zhang Y, Qiu D, Jin Z, Su C, Peng C, Wang JH, Dong MQ, Wan Y, Ma J, Cheng H, Huang Y, Yu Y. A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation. Nat Cell Biol. 2019 Oct;21(10):1261-1272. doi: 10.1038/s41556-019-0396-0. Epub, 2019 Sep 30. PMID:31570835 doi:http://dx.doi.org/10.1038/s41556-019-0396-0

6iew, resolution 1.50Å

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