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| <StructureSection load='5yim' size='340' side='right'caption='[[5yim]], [[Resolution|resolution]] 3.39Å' scene=''> | | <StructureSection load='5yim' size='340' side='right'caption='[[5yim]], [[Resolution|resolution]] 3.39Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5yim]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YIM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YIM FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5yim]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YIM FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5yij|5yij]], [[5yik|5yik]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.394Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5yim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yim OCA], [http://pdbe.org/5yim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yim RCSB], [http://www.ebi.ac.uk/pdbsum/5yim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yim ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yim OCA], [https://pdbe.org/5yim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yim RCSB], [https://www.ebi.ac.uk/pdbsum/5yim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yim ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q6RCR0_LEGPN Q6RCR0_LEGPN] |
| Protein ubiquitination is a multifaceted post-translational modification that controls almost every process in eukaryotic cells. Recently, the Legionella effector SdeA was reported to mediate a unique phosphoribosyl-linked ubiquitination through successive modifications of the Arg42 of ubiquitin (Ub) by its mono-ADP-ribosyltransferase (mART) and phosphodiesterase (PDE) domains. However, the mechanisms of SdeA-mediated Ub modification and phosphoribosyl-linked ubiquitination remain unknown. Here we report the structures of SdeA in its ligand-free, Ub-bound and Ub-NADH-bound states. The structures reveal that the mART and PDE domains of SdeA form a catalytic domain over its C-terminal region. Upon Ub binding, the canonical ADP-ribosyltransferase toxin turn-turn (ARTT) and phosphate-nicotinamide (PN) loops in the mART domain of SdeA undergo marked conformational changes. The Ub Arg72 might act as a 'probe' that interacts with the mART domain first, and then movements may occur in the side chains of Arg72 and Arg42 during the ADP-ribosylation of Ub. Our study reveals the mechanism of SdeA-mediated Ub modification and provides a framework for further investigations into the phosphoribosyl-linked ubiquitination process.
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| Structural basis of ubiquitin modification by the Legionella effector SdeA.,Dong Y, Mu Y, Xie Y, Zhang Y, Han Y, Zhou Y, Wang W, Liu Z, Wu M, Wang H, Pan M, Xu N, Xu CQ, Yang M, Fan S, Deng H, Tan T, Liu X, Liu L, Li J, Wang J, Fang X, Feng Y Nature. 2018 May;557(7707):674-678. doi: 10.1038/s41586-018-0146-7. Epub 2018 May, 23. PMID:29795342<ref>PMID:29795342</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5yim" style="background-color:#fffaf0;"></div>
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| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Atcc 33152]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Dong, Y]] | | [[Category: Legionella pneumophila]] |
| [[Category: Feng, Y]] | | [[Category: Dong Y]] |
| [[Category: Wang, W]] | | [[Category: Feng Y]] |
| [[Category: Monomer]] | | [[Category: Wang W]] |
| [[Category: Multi-domain protein]]
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| [[Category: Transferase]]
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