1rao: Difference between revisions

Revision as of 07:16, 3 May 2008

CRYSTAL STRUCTURE OF A TERNARY COMPLEX OF E. COLI HPPK WITH AMP AND 6-HYDROXYMETHYLPTERIN-DIPHOSPHATE AT 1.56 ANGSTROM RESOLUTION

OverviewOverview

6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the Mg(2+)-dependent pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP). The reaction follows a bi-bi mechanism with ATP as the first substrate and AMP and HP pyrophosphate (HPPP) as the two products. HPPK is a key enzyme in the folate biosynthetic pathway and is essential for microorganisms but absent from mammals. For the HPPK-catalyzed pyrophosphoryl transfer, a reaction coordinate is constructed on the basis of the thermodynamic and transient kinetic data we reported previously, and the reaction trajectory is mapped out with five three-dimensional structures of the enzyme at various liganded states. The five structures are apo-HPPK (ligand-free enzyme), HPPK.MgATP(analog) (binary complex of HPPK with its first substrate) and HPPK.MgATP(analog).HP (ternary complex of HPPK with both substrates), which we reported previously, and HPPK.AMP.HPPP (ternary complex of HPPK with both product molecules) and HPPK.HPPP (binary complex of HPPK with one product), which we present in this study.

About this StructureAbout this Structure

1RAO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Reaction trajectory of pyrophosphoryl transfer catalyzed by 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase., Blaszczyk J, Shi G, Li Y, Yan H, Ji X, Structure. 2004 Mar;12(3):467-75. PMID:15016362 Page seeded by OCA on Sat May 3 07:16:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1rao.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1rao |SIZE=350|CAPTION= <scene name='initialview01'>1rao</scene>, resolution 1.56&Aring;
+The line below this paragraph, containing "STRUCTURE_1rao", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=HH2:6-HYDROXYMETHYLPTERIN-DIPHOSPHATE'>HH2</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= FOLK, B0142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1rao|  PDB=1rao  |  SCENE=  }}
-|RELATEDENTRY=[[1hka|1HKA]], [[1eqm|1EQM]], [[1eq0|1EQ0]], [[1q0n|1Q0N]], [[1ex8|1EX8]], [[1cbk|1CBK]], [[1dy3|1DY3]], [[1f9y|1F9Y]], [[1f9h|1F9H]], [[1g4c|1G4C]], [[1hq2|1HQ2]], [[1im6|1IM6]], [[1kbr|1KBR]], [[1rb0|1RB0]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rao OCA], [http://www.ebi.ac.uk/pdbsum/1rao PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rao RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF A TERNARY COMPLEX OF E. COLI HPPK WITH AMP AND 6-HYDROXYMETHYLPTERIN-DIPHOSPHATE AT 1.56 ANGSTROM RESOLUTION'''
@@ Line 30: / Line 27: @@
 [[Category: 6-hydroxymethyl-7,8-dihydropterin]]
 [[Category: 6-hydroxymethylpterin]]
-[[Category: antimicrobial agent]]
+[[Category: Antimicrobial agent]]
-[[Category: catalytic mechanism]]
+[[Category: Catalytic mechanism]]
-[[Category: drug design]]
+[[Category: Drug design]]
-[[Category: folate]]
+[[Category: Folate]]
-[[Category: hppk]]
+[[Category: Hppk]]
-[[Category: product release]]
+[[Category: Product release]]
-[[Category: pterin]]
+[[Category: Pterin]]
-[[Category: pyrophosphokinase]]
+[[Category: Pyrophosphokinase]]
-[[Category: pyrophosphoryl transfer]]
+[[Category: Pyrophosphoryl transfer]]
-[[Category: substrate specificity]]
+[[Category: Substrate specificity]]
-[[Category: ternary complex]]
+[[Category: Ternary complex]]
-[[Category: x-ray crystallography]]
+[[Category: X-ray crystallography]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:16:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:25:31 2008''