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==Solution structure of the a-subunit of human chorionic gonadotropin [modeled without carbohydrate residues]==
==Solution structure of the a-subunit of human chorionic gonadotropin [modeled without carbohydrate residues]==
<StructureSection load='1dz7' size='340' side='right'caption='[[1dz7]], [[NMR_Ensembles_of_Models | 27 NMR models]]' scene=''>
<StructureSection load='1dz7' size='340' side='right'caption='[[1dz7]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1dz7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DZ7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1dz7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DZ7 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hcn|1hcn]], [[1hrp|1hrp]], [[1xul|1xul]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dz7 OCA], [https://pdbe.org/1dz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dz7 RCSB], [https://www.ebi.ac.uk/pdbsum/1dz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dz7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dz7 OCA], [https://pdbe.org/1dz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dz7 RCSB], [https://www.ebi.ac.uk/pdbsum/1dz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dz7 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GLHA_HUMAN GLHA_HUMAN]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dz7 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dz7 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional solution structure of the alpha-subunit in the alpha, beta heterodimeric human chorionic gonadotropin (hCG), deglycosylated with endo-beta-N-acetylglucosaminidase-B (dg-alpha hCG), was determined using 2D homonuclear and 2D heteronuclear 1H, 13C NMR spectroscopy at natural abundance in conjunction with the program package XPLOR. The distance geometry/simulated annealing protocol was modified to allow for the efficient modelling of the cystine knot motif present in alpha hCG. The protein structure was modelled with 620 interproton distance restraints and the GlcNAc residue linked to Asn78 was modelled with 30 protein-carbohydrate and 3 intraresidual NOEs. The solution structure of dg-alpha hCG is represented by an ensemble of 27 structures. In comparison to the crystal structure of the dimer, the solution structure of free dg-alpha hCG exhibits: (a) an increased structural disorder (residues 33-57); (b) a different backbone conformation near Val76 and Glu77; and (c) a larger flexibility. These differences are caused by the absence of the interactions with the beta-subunit. Consequently, in free dg-alpha hCG, compared to the intact dimer, the two hairpin loops 20-23 and 70-74 are arranged differently with respect to each other. The beta-GlcNAc(78) is tightly associated with the hydrophobic protein-core in between the beta-hairpins. This conclusion is based on the NOEs from the axial H1, H3, H5 atoms and the N-acetyl protons of beta-GlcNAc(78) to the protein-core. The hydrophobic protein-core between the beta-hairpins is thereby shielded from the solvent.
Solution structure of the alpha-subunit of human chorionic gonadotropin.,Erbel PJ, Karimi-Nejad Y, De Beer T, Boelens R, Kamerling JP, Vliegenthart JF Eur J Biochem. 1999 Mar;260(2):490-8. PMID:10095786<ref>PMID:10095786</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1dz7" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Hormone|Hormone]]
*[[Hormone|Hormone]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Beer, T De]]
[[Category: Boelens R]]
[[Category: Boelens, R]]
[[Category: De Beer T]]
[[Category: Erbel, P J.A]]
[[Category: Erbel PJA]]
[[Category: Kamerling, J P]]
[[Category: Kamerling JP]]
[[Category: Karimi-Nejad, Y]]
[[Category: Karimi-Nejad Y]]
[[Category: Vliegenthart, J F.G]]
[[Category: Vliegenthart JFG]]
[[Category: Chorionic gonadotropin]]
[[Category: Chorionic gonadotropin free a subunit]]
[[Category: Cystine knot]]
[[Category: Glycoprotein]]
[[Category: Glycoprotein structure]]
[[Category: Xplor]]

Revision as of 12:55, 20 March 2024

Solution structure of the a-subunit of human chorionic gonadotropin [modeled without carbohydrate residues]Solution structure of the a-subunit of human chorionic gonadotropin [modeled without carbohydrate residues]

Structural highlights

1dz7 is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLHA_HUMAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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