6u9f: Difference between revisions

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<SX load='6u9f' size='340' side='right' viewer='molstar' caption='[[6u9f]], [[Resolution|resolution]] 4.35&Aring;' scene=''>
<SX load='6u9f' size='340' side='right' viewer='molstar' caption='[[6u9f]], [[Resolution|resolution]] 4.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6u9f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Fratn Fratn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9F FirstGlance]. <br>
<table><tr><td colspan='2'>[[6u9f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_U112 Francisella tularensis subsp. novicida U112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9F FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pdpA, FTN_1309 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=401614 FRATN]), FTN_1312 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=401614 FRATN])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.35&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9f OCA], [https://pdbe.org/6u9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9f RCSB], [https://www.ebi.ac.uk/pdbsum/6u9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9f ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9f OCA], [https://pdbe.org/6u9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9f RCSB], [https://www.ebi.ac.uk/pdbsum/6u9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9f ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0Q7H0_FRATN A0Q7H0_FRATN]
Francisella bacteria rely on a phylogenetically distinct type VI secretion system (T6SS) to escape host phagosomes and cause the fatal disease tularemia, but the structural and molecular mechanisms involved are unknown. Here we report the atomic structure of the Francisella T6SS central spike complex, obtained by cryo-electron microscopy. Our structural and functional studies demonstrate that, unlike the single-protein spike composition of other T6SS subtypes, Francisella T6SS's central spike is formed by two proteins, PdpA and VgrG, akin to T4-bacteriophage gp27 and gp5, respectively, and that PdpA has unique characteristics, including a putative cargo within its cavity and an N-terminal helical lid. Structure-guided mutagenesis demonstrates that the PdpA N-terminal lid and C-terminal spike are essential to Francisella T6SS function. PdpA is thus both an adaptor, connecting VgrG to the tube, and a likely carrier of secreted cargo. These findings are important to understanding Francisella pathogenicity and designing therapeutics to combat tularemia.
 
Atomic Structure of the Francisella T6SS Central Spike Reveals a Unique alpha-Helical Lid and a Putative Cargo.,Yang X, Clemens DL, Lee BY, Cui Y, Zhou ZH, Horwitz MA Structure. 2019 Dec 3;27(12):1811-1819.e6. doi: 10.1016/j.str.2019.10.007. Epub, 2019 Oct 31. PMID:31677891<ref>PMID:31677891</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6u9f" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Fratn]]
[[Category: Francisella tularensis subsp. novicida U112]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Clemens, D L]]
[[Category: Clemens DL]]
[[Category: Cui, Y X]]
[[Category: Cui YX]]
[[Category: Horwitz, M A]]
[[Category: Horwitz MA]]
[[Category: Lee, B Y]]
[[Category: Lee B-Y]]
[[Category: Yang, X]]
[[Category: Yang X]]
[[Category: Zhou, Z H]]
[[Category: Zhou ZH]]
[[Category: Complex]]
[[Category: Transport protein]]
[[Category: Type vi secretion system]]

Latest revision as of 12:29, 20 March 2024

Structure of Francisella PdpA-VgrG Complex, LiddedStructure of Francisella PdpA-VgrG Complex, Lidded

6u9f, resolution 4.35Å

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