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| <SX load='6ouu' size='340' side='right' viewer='molstar' caption='[[6ouu]], [[Resolution|resolution]] 4.10Å' scene=''> | | <SX load='6ouu' size='340' side='right' viewer='molstar' caption='[[6ouu]], [[Resolution|resolution]] 4.10Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6ouu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Norovirus_hu/gii.4/minerva/2006/usa Norovirus hu/gii.4/minerva/2006/usa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OUU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OUU FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6ouu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Norovirus_Hu/GII.4/Minerva/2006/USA Norovirus Hu/GII.4/Minerva/2006/USA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OUU FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6otf|6otf]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ouu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ouu OCA], [http://pdbe.org/6ouu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ouu RCSB], [http://www.ebi.ac.uk/pdbsum/6ouu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ouu ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ouu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ouu OCA], [https://pdbe.org/6ouu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ouu RCSB], [https://www.ebi.ac.uk/pdbsum/6ouu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ouu ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/R4I3T2_9CALI R4I3T2_9CALI] |
| Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available in detail for only a single strain (GI.1). We present high-resolution (2.6- to 4.1-A) cryoelectron microscopy (cryo-EM) structures of GII.4, GII.2, GI.7, and GI.1 human norovirus outbreak strain virus-like particles (VLPs). Although norovirus VLPs have been thought to exist in a single-sized assembly, our structures reveal polymorphism between and within genogroups, with small, medium, and large particle sizes observed. Using asymmetric reconstruction, we were able to resolve a Zn(2+) metal ion adjacent to the coreceptor binding site, which affected the structural stability of the shell. Our structures serve as valuable templates for facilitating vaccine formulations.
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| High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations.,Jung J, Grant T, Thomas DR, Diehnelt CW, Grigorieff N, Joshua-Tor L Proc Natl Acad Sci U S A. 2019 Jun 10. pii: 1903562116. doi:, 10.1073/pnas.1903562116. PMID:31182604<ref>PMID:31182604</ref>
| | ==See Also== |
| | | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 6ouu" style="background-color:#fffaf0;"></div>
| |
| == References == | |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Norovirus hu/gii 4/minerva/2006/usa]] | | [[Category: Norovirus Hu/GII 4/Minerva/2006/USA]] |
| [[Category: Diehnelt, C W]] | | [[Category: Diehnelt CW]] |
| [[Category: Grant, T]] | | [[Category: Grant T]] |
| [[Category: Grigorieff, N]] | | [[Category: Grigorieff N]] |
| [[Category: Joshua-Tor, L]] | | [[Category: Joshua-Tor L]] |
| [[Category: Jung, J]] | | [[Category: Jung J]] |
| [[Category: Thomas, D R]] | | [[Category: Thomas DR]] |
| [[Category: Caliciviridae]]
| |
| [[Category: Calicivirus]]
| |
| [[Category: Gii 4]]
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| [[Category: Norovirus]]
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| [[Category: Virus like particle]]
| |